Betaine chloride

Name: Betaine chloride
Brand: MedChemExpress
SKU: HY-N0739
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0739 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Betaine hydrochloride is a natural compound found in many foods and also an active methyl-donor which can maintain normal DNA methylation patterns.

For research use only. We do not sell to patients.

Betaine chloride Chemical Structure

CAS No. : 590-46-5

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100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Betaine chloride:

Betaine In-stock

3 Publications Citing Use of MCE Betaine chloride

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Betaine hydrochloride is a natural compound found in many foods and also an active methyl-donor which can maintain normal DNA methylation patterns.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

In Vitro

It is found that proliferation of HeLa cells is inhibited significantly upon exposure to increasing Betaine hydrochloride levels with the MTT test (p<0.05). The percentage of S phase cells in the low dose groups (<5 mg/mL) are distinctly higher than in high dose groups, and the rates of Sub-G1 phase are the opposite (p<0.05). A high concentration of Betaine hydrochloride (>5.0 mg/mL) significantly promotes the apoptosis of HeLa cells (p<0.01). SOD activities of the low dose groups are slightly higher than the control group (p<0.05), and there are obvious synchronicity and correlation among the expression of promoting apoptosis genes Bax, P53, Caspase 3 and apoptosis suppression gene Bcl-2. In response to an apoptosis-inducing stimulus, p53 and cyclin D1 can be activated with blockage of the cell cycle at G1/S or S/G2 checkpoints^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Liver-to-body weight ratio is attenuated by Betaine hydrochloride supplementation. When Betaine hydrochloride is added to the alcohol-containing liquid diet, both hepatic triglyceride (TG) accumulation and liver injury are significantly reduced. Betaine hydrochloride supplementation attenuates epididymal fat pad mass loss induced by chronic alcohol feeding. Betaine hydrochloride supplementation prevents the depletion of S-adenosylhomocysteine (SAM) and reduces the increase in S-adenosylhomocysteine (SAH), thereby improving the methylation state of the adipose tissue^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

153.61

Formula

C₅H₁₂ClNO₂

CAS No.

590-46-5

Appearance

Solid

Color

White to off-white

SMILES

C[N+](C)(C)CC(O)=O.[Cl-]

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (651.00 mM; Need ultrasonic)

DMSO : 5 mg/mL (32.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.5100 mL	32.5500 mL	65.0999 mL
5 mM	1.3020 mL	6.5100 mL	13.0200 mL
10 mM	0.6510 mL	3.2550 mL	6.5100 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (276 KB) SDS (416 KB)

COA (247 KB) HNMR (173 KB)

Handling Instructions (2659 KB)

References

[1]. Guo Y, et al. Betaine Effects on Morphology, Proliferation, and p53-induced Apoptosis of HeLa Cervical Carcinoma Cells in Vitro. Asian Pac J Cancer Prev. 2015;16(8):3195-201. [Content Brief]

[2]. Dou X, et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014 Sep;171(17):4073-86. [Content Brief]

Cell Assay ^[1]	Cytotoxic effects of Betaine hydrochloride are evaluated by MTT assays. Briefly, 5×10³ HeLa or MCF-10A cells/well are seeded in a 96-well plate and cultured overnight. Then, different concentrations of Betaine hydrochloride (from 0 to 100 mg/mL) are added to each well, and the MTT assay is performed at 24, 48, 72 and 96 h after incubation. Cu/Zn-SOD activities are detected using the superoxide dismutase Assay Kit according to the manufacturer’s instructions^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Male C57BL/6 mice weighing 25±0.5 g are used in this study. Animals are randomly assigned to four groups (n=6 to 8 per group) and pair-fed a modified Lieber-DeCarli alcohol (AF) or isocaloric maltose dextrin control liquid diet (PF) for 5 weeks with a stepwise feeding procedure. The ethanol content starts at 30% of total calories and gradually increases up to 36% in the last week. Betaine hydrochloride is administered as a supplement in the liquid diets (both PF and AF) at a concentration of 0.5% (w/v), simultaneously with the alcohol. All animals have access to diet and water ad libitum. At the end of the experiment, the mice are killed after being deprived of food for 4 h, and the plasma, liver and epididymal fat pad samples are harvested for assays^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Guo Y, et al. Betaine Effects on Morphology, Proliferation, and p53-induced Apoptosis of HeLa Cervical Carcinoma Cells in Vitro. Asian Pac J Cancer Prev. 2015;16(8):3195-201. [Content Brief] [2]. Dou X, et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014 Sep;171(17):4073-86. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	6.5100 mL	32.5500 mL	65.0999 mL	162.7498 mL
	5 mM	1.3020 mL	6.5100 mL	13.0200 mL	32.5500 mL
	10 mM	0.6510 mL	3.2550 mL	6.5100 mL	16.2750 mL
	15 mM	0.4340 mL	2.1700 mL	4.3400 mL	10.8500 mL
	20 mM	0.3255 mL	1.6275 mL	3.2550 mL	8.1375 mL
	25 mM	0.2604 mL	1.3020 mL	2.6040 mL	6.5100 mL
	30 mM	0.2170 mL	1.0850 mL	2.1700 mL	5.4250 mL
H₂O	40 mM	0.1627 mL	0.8137 mL	1.6275 mL	4.0687 mL
	50 mM	0.1302 mL	0.6510 mL	1.3020 mL	3.2550 mL
	60 mM	0.1085 mL	0.5425 mL	1.0850 mL	2.7125 mL
	80 mM	0.0814 mL	0.4069 mL	0.8137 mL	2.0344 mL
	100 mM	0.0651 mL	0.3255 mL	0.6510 mL	1.6275 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.