2. PI3K/Akt/mTOR
  3. Akt
  4. Crosstide

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Crosstide Chemical Structure

Crosstide Chemical Structure

CAS No. : 171783-05-4

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5 mg USD 528 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Crosstide:

Crosstide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Crosstide

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.

In Vitro

16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Crosstide Related Antibodies
Molecular Weight

1164.23

Formula

C48H77N17O17
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly
Sequence Shortening

GRPRTSSFAEG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (42.95 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8589 mL 4.2947 mL 8.5894 mL
5 mM 0.1718 mL 0.8589 mL 1.7179 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.76%

SDS (252 KB)

COA (248 KB) LCMS (87 KB) Elemental Analysis Report (107 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.8589 mL 4.2947 mL 8.5894 mL 21.4734 mL
5 mM 0.1718 mL 0.8589 mL 1.7179 mL 4.2947 mL
10 mM 0.0859 mL 0.4295 mL 0.8589 mL 2.1473 mL
15 mM 0.0573 mL 0.2863 mL 0.5726 mL 1.4316 mL
20 mM 0.0429 mL 0.2147 mL 0.4295 mL 1.0737 mL
25 mM 0.0344 mL 0.1718 mL 0.3436 mL 0.8589 mL
30 mM 0.0286 mL 0.1432 mL 0.2863 mL 0.7158 mL
40 mM 0.0215 mL 0.1074 mL 0.2147 mL 0.5368 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Crosstide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Crosstide171783-05-4AktPKBProtein kinase BInhibitorinhibitorinhibit

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