2. GPCR/G Protein Neuronal Signaling
  3. Opioid Receptor
  4. BW-180C

BW-180C  (Synonyms: [D-Ala2, D-Leu5]-Enkephalin; DADLE)

Cat. No.: HY-105343 Purity: 99.92%
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BW-180C ([D-Ala2, D-Leu5]-Enkephalin; DADLE) is an δ opioid receptor (DOR) agonist, which belongs to the enkephalin family. Neuroprotective agent. BW-180C reversibly inhibits cellular transcription in neurons without causing cell injury.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

BW-180C Chemical Structure

BW-180C Chemical Structure

CAS No. : 63631-40-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 In-stock
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Solid
5 mg USD 70 In-stock
10 mg USD 110 In-stock
25 mg USD 190 In-stock
50 mg USD 350 In-stock
100 mg USD 620 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

BW-180C Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BW-180C

View All Opioid Receptor Isoform Specific Products:

View All Isoforms
μ Opioid Receptor/MOR κ Opioid Receptor/KOR δ Opioid Receptor/DOR NOP Receptor/ORL1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BW-180C ([D-Ala2, D-Leu5]-Enkephalin; DADLE) is an δ opioid receptor (DOR) agonist, which belongs to the enkephalin family. Neuroprotective agent. BW-180C reversibly inhibits cellular transcription in neurons without causing cell injury[1].

IC50 & Target

Opioid Receptor[1]
Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
1.3 μM
Compound: DADLE
Agonist activity at GFP-fused mu opioid receptor (unknown origin) expressed in HEK293 cells assessed as recruitment of beta-arrestin-2 after 10 mins by BRET assay
Agonist activity at GFP-fused mu opioid receptor (unknown origin) expressed in HEK293 cells assessed as recruitment of beta-arrestin-2 after 10 mins by BRET assay
[PMID: 24613457]
HEK293 EC50
54 nM
Compound: DADLE
Agonist activity at GFP-fused delta opioid receptor (unknown origin) expressed in HEK293 cells assessed as recruitment of beta-arrestin-2 after 10 mins by BRET assay
Agonist activity at GFP-fused delta opioid receptor (unknown origin) expressed in HEK293 cells assessed as recruitment of beta-arrestin-2 after 10 mins by BRET assay
[PMID: 24613457]
In Vitro

"Pretreatment with BW-180C (DADLE) dose-dependently enhances survival of neurons in the central nervous system. BW-180C is able to induce a reversible hibernation-like state in HeLa cells, a cell line derived from cervical cancer cells, by inhibiting transcription and proliferation in these cells[1].
BW-180C (100 pM to 10 μM) inhibits cellular transcription by ~50% by 24 h compared with vehicle-treated controls[1].
BW-180C does not affect total RNA polymerase II expression. DADLE inhibits phosphorylation of RNA polymerase II in primary cortical neurons[1]. "

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BW-180C Related Antibodies

Cell Viability Assay[1]

Cell Line: Human neuroblastoma cells, SH-SY5Y cells
Concentration: 100 pM to 10 μM
Incubation Time: 24-72 hours
Result: Significantly inhibited cellular transcription.

Western Blot Analysis[1]

Cell Line: Primary cortical neurons
Concentration: 100 nM
Incubation Time: 72 hours
Result: Treatment significantly decreased phosphorylation of RNA polymerase II at both Ser 2 and Ser 5.
In Vivo

BW-180C ( [D-Ala2, D-Leu5]-Enkephalin; DADLE) protects liver against ischemia-reperfusion injury in the rat. DADLE (5 mg/kg) protects the heart, lung, and jejunum against ischemia-reperfusion (I-R) injury[2].
BW-180C (DADLE) improves hepatic ischemia/reperfusion injury in mice via activation of the Nrf2/HO1 pathway[3].
DADLE (5 mg/kg) significantly reduces the levels of alanine aminotransferase and aspartate aminotransferase in the serum, and the levels of malondialdehyde in the liver homogenate[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats weighing 200 to 250 g[2]
Dosage: 5 mg/kg
Administration: Administered intravenously into the inferior vena cava
Result: After 120 min of reperfusion, the serum GPT levels were significantly lower in the DADLE group than in the Control group.
Animal Model: Male C57BL/6 mice (age, 35-42 days; weight, 18-22 g; 6 mice in each group)[3]
Dosage: 5 mg/kg
Administration: Injected intraperitoneally 15 min prior to I/R
Result: The serum levels of AST and ALT in the serum DADLE group were significantly lower.
Molecular Weight

569.65

Formula

C29H39N5O7
CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (438.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7555 mL 8.7773 mL 17.5546 mL
5 mM 0.3511 mL 1.7555 mL 3.5109 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.92%

SDS (252 KB)

COA (247 KB) HNMR (131 KB) LCMS (86 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7555 mL 8.7773 mL 17.5546 mL 43.8866 mL
5 mM 0.3511 mL 1.7555 mL 3.5109 mL 8.7773 mL
10 mM 0.1755 mL 0.8777 mL 1.7555 mL 4.3887 mL
15 mM 0.1170 mL 0.5852 mL 1.1703 mL 2.9258 mL
20 mM 0.0878 mL 0.4389 mL 0.8777 mL 2.1943 mL
25 mM 0.0702 mL 0.3511 mL 0.7022 mL 1.7555 mL
30 mM 0.0585 mL 0.2926 mL 0.5852 mL 1.4629 mL
40 mM 0.0439 mL 0.2194 mL 0.4389 mL 1.0972 mL
50 mM 0.0351 mL 0.1755 mL 0.3511 mL 0.8777 mL
60 mM 0.0293 mL 0.1463 mL 0.2926 mL 0.7314 mL
80 mM 0.0219 mL 0.1097 mL 0.2194 mL 0.5486 mL
100 mM 0.0176 mL 0.0878 mL 0.1755 mL 0.4389 mL
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BW-180C Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BW-180C63631-40-3[D-Ala2, D-Leu5]-Enkephalin DADLEOpioid ReceptorNeuronsSH-SY5YTranscriptionDetlaδopioidDORencephalinNeuroprotectiveischemiareperfusioninjuryInhibitorinhibitorinhibit

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