2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. EGF Receptor Substrate 2 (Phospho-Tyr5)

EGF Receptor Substrate 2 (Phospho-Tyr5) 

Cat. No.: HY-P0320 Purity: 99.90%
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EGF Receptor Substrate 2 (Phospho-Tyr5) acetate, a biologically active peptide, is a tyrosine phosphate substrate. EGF Receptor Substrate 2 (Phospho-Tyr5) acetate can be used to detect protein tyrosine phosphatases activity.

For research use only. We do not sell to patients.

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EGF Receptor Substrate 2 (Phospho-Tyr5) Chemical Structure

EGF Receptor Substrate 2 (Phospho-Tyr5) Chemical Structure

CAS No. : 149261-42-7

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1 mg USD 108 In-stock
5 mg USD 312 In-stock
10 mg USD 552 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of EGF Receptor Substrate 2 (Phospho-Tyr5):

EGF Receptor Substrate 2 (Phospho-Tyr5) Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

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Description

EGF Receptor Substrate 2 (Phospho-Tyr5) acetate, a biologically active peptide, is a tyrosine phosphate substrate. EGF Receptor Substrate 2 (Phospho-Tyr5) acetate can be used to detect protein tyrosine phosphatases activity[1].

Molecular Weight

1328.28

Formula

C54H82N13O24P
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

H-Asp-Ala-Asp-Glu-{pTyr}-Leu-Ile-Pro-Gln-Gln-Gly
Sequence Shortening

DADE-{pTyr}-LIPQQG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (37.64 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7529 mL 3.7643 mL 7.5285 mL
5 mM 0.1506 mL 0.7529 mL 1.5057 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Kinase Assay
[1]

Briefly, the assays are performed in 50 μL volumes in a microtiter plate using the generalized PTPase substrates ENDpYINASL and DADEpYLIPQQG (where pY represents phosphotyrosine) and assay buffer containing 25 mM Tris-HCl (pH 7.4), 50 mM NaCl, 2 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 1 mg of bovine serum albumin/mL. The reactions are started by the addition of 5 μg of either CAV GST-VP2, GST, CAV GST-VP2 containing the C95S mutation, or TLMV GST-ORF2 or 2 units of the positive control T cell protein-tyrosine phosphatase (TC-PTP) in assay buffer. Control reactions are assayed with either ENDpYINASL substrate alone, DADEpYLIPQQG substrate alone, CAV GST-VP2 without substrate, CAV GST-VP2 containing the C95S mutation without substrate, TLMV GST-ORF2 without substrate, TC-PTP without substrate, or assay buffer with neither enzyme nor substrate. A phosphate standard curve is derived using a supplied phosphate standard. The reactions are terminated by the addition of the malachite green detection reagent.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.7529 mL 3.7643 mL 7.5285 mL 18.8213 mL
5 mM 0.1506 mL 0.7529 mL 1.5057 mL 3.7643 mL
10 mM 0.0753 mL 0.3764 mL 0.7529 mL 1.8821 mL
15 mM 0.0502 mL 0.2510 mL 0.5019 mL 1.2548 mL
20 mM 0.0376 mL 0.1882 mL 0.3764 mL 0.9411 mL
25 mM 0.0301 mL 0.1506 mL 0.3011 mL 0.7529 mL
30 mM 0.0251 mL 0.1255 mL 0.2510 mL 0.6274 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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EGF Receptor Substrate 2 (Phospho-Tyr5) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EGF Receptor Substrate 2 (Phospho-Tyr5)149261-42-7PhosphataseInhibitorinhibitorinhibit

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