Episilvestrol

Cat. No.: HY-15359 Purity: 99.86%: FAQs
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Episilvestrol is a derivative of silvestrol, isolated from the fruits and twigs of Aglaia perviridis, and is a specific eIF4A-targeting translation inhibitor, with antitumor activity.

For research use only. We do not sell to patients.

Episilvestrol Chemical Structure

CAS No. : 697235-39-5

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1 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
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Other Forms of Episilvestrol:

Silvestrol aglycone In-stock
Silvestrol In-stock

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Description

Episilvestrol is a derivative of silvestrol, isolated from the fruits and twigs of Aglaia perviridis, and is a specific eIF4A-targeting translation inhibitor, with antitumor activity.

IC₅₀ & Target

eIF4

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	> 20 μM Compound: 7	Inhibition of NF-kappaB p65 isolated from nuclear extract of human HeLa cells assessed as blockade of binding to biotinylated consesus sequence by chemiluminescence assay Inhibition of NF-kappaB p65 isolated from nuclear extract of human HeLa cells assessed as blockade of binding to biotinylated consesus sequence by chemiluminescence assay	[PMID: 20939540]
HeLa	IC₅₀	0.02 μM Compound: 8	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition by [35S] methionine incorporation assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition by [35S] methionine incorporation assay	[PMID: 33592144]
HL-60	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
HT-29	ED₅₀	0.0007 μM Compound: 7	Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay	[PMID: 20939540]
HT-29	IC₅₀	0.004 μM Compound: Silvestrol	Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 21513293]
Kasumi 1	IC₅₀	24 nM Compound: Silvestrol	Cytotoxicity against human Kasumi-1 cells incubated for 48 hrs by MTS assay Cytotoxicity against human Kasumi-1 cells incubated for 48 hrs by MTS assay	[PMID: 27983842]
LNCaP	ED₅₀	1.5 nM Compound: 13	Growth inhibition of human LNCAP cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test Growth inhibition of human LNCAP cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test	[PMID: 19161316]
Lu1	ED₅₀	1.2 nM Compound: 13	Growth inhibition of human Lu1 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test Growth inhibition of human Lu1 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test	[PMID: 19161316]
MCF7	ED₅₀	1.5 nM Compound: 13	Growth inhibition of human MCF7 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test Growth inhibition of human MCF7 cells xenografted in ip dosed NCr nu/nu mouse administered once daily from day 3 to 6 after implantation measured on day 7 by MTT-based hollow-fiber test	[PMID: 19161316]
MDA-MB-231	EC₅₀	0.8 nM Compound: 1	Inhibition of eIF4FA in human MDA-MB-231 cells transfected with c-myc- 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay Inhibition of eIF4FA in human MDA-MB-231 cells transfected with c-myc- 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay	[PMID: 23025805]
MDA-MB-231	EC₅₀	3 nM Compound: 1	Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTS assay Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTS assay	[PMID: 23025805]
MDA-MB-231	EC₅₀	7 nM Compound: 1	Inhibition of eIF4FA in human MDA-MB-231 cells transfected with tubulin 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay Inhibition of eIF4FA in human MDA-MB-231 cells transfected with tubulin 5'-UTR-luciferase construct assessed as reduction in translation initiation incubated for 24 hrs by differential translation assay	[PMID: 23025805]
MDA-MB-435	IC₅₀	0.006 μM Compound: Silvestrol	Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTS assay Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTS assay	[PMID: 21513293]
MV4-11	IC₅₀	11 nM Compound: Silvestrol	Cytotoxicity against human MV4-11 cells incubated for 48 hrs by MTS assay Cytotoxicity against human MV4-11 cells incubated for 48 hrs by MTS assay	[PMID: 27983842]
NB-4	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human NB-4 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human NB-4 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
OCI-AML2	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human OCI-AML2 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human OCI-AML2 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
OCI-AML-3	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human OCI-AML-3 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human OCI-AML-3 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]
U-937	IC₅₀	4 nM Compound: 8	Antiproliferative activity against human U-937 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay Antiproliferative activity against human U-937 cells assessed as cell growth inhibition incubated for 48 hrs by MTS assay	[PMID: 33592144]

In Vitro

Episilvestrol is a specific eIF4A-targeting translation inhibitor, with antitumor activity^[1]. Episilvestrol is cytotoxic activity against several human cancer cell lines, such as Lu1, LNCaP, MCF-7 and HUVEC cells, with ED₅₀s of 3.8, 3.8, 5.5 and 15.3 nM, respectively^[2]. The GI₅₀s of Episilvestrol against the cell proliferation of NCI-H460 and MCF-7 cels are 17.96 nM and 17.96 nM after first test and 15.6 nM and 18.7 nM after 2 months via SRB assay. Episilvestrol also suppresses HK1 cells and EBV-positive C666.1 NPC cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

654.66

Formula

C₃₄H₃₈O₁₃

CAS No.

697235-39-5

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]([C@H]1C2=CC=CC=C2)[C@@H](O)[C@]3(O)[C@@]1(C4=CC=C(OC)C=C4)OC5=CC(O[C@@H]6O[C@@H]([C@@H](O)CO)CO[C@H]6OC)=CC(OC)=C35)OC

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (38.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5275 mL	7.6376 mL	15.2751 mL
5 mM	0.3055 mL	1.5275 mL	3.0550 mL
10 mM	0.1528 mL	0.7638 mL	1.5275 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.86%

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Data Sheet (280 KB) SDS (252 KB)

COA (259 KB) RP-HPLC (223 KB)

Handling Instructions (2659 KB)

References

[1]. Chambers JM, et al. Synthesis of biotinylated episilvestrol: highly selective targeting of the translation factors eIF4AI/II. Org Lett. 2013 Mar 15;15(6):1406-9. [Content Brief]

[2]. Hwang BY, et al. Silvestrol and episilvestrol, potential anticancer rocaglate derivatives from Aglaia silvestris. J Org Chem. 2004 May 14;69(10):3350-8. [Content Brief]

[3]. Daker M, et al. Inhibition of nasopharyngeal carcinoma cell proliferation and synergism of cisplatin with silvestrol and episilvestrol isolated from Aglaia stellatopilosa. Exp Ther Med. 2016 Jun;11(6):2117-2126. Epub 2016 Mar 29. [Content Brief]

Cell Assay
^[3]

A total of 1×10⁴ HK1 cells/well or 3×10⁴ C666.1 cells/well are seeded into 96-multiwell microtiter plates. At 24 h following seeding, the medium is aspirated and replaced with fresh medium containing various concentrations of silvestrol or Episilvestrol. Vehicle control cultures receive DMSO alone. The cells are then incubated for 24 h at 37°C in an atmosphere containing 5% CO₂. The number of viable cells at the end of the incubation period is measured using MTS assay. Absorbance at 490 nm is read and subtracted with non-specific absorbance measured at 630 nm. Wells containing medium without cells serve as blanks. Cell viability is calculated as a percentage compared to the control cells, which are arbitrarily assigned 100% viability. The half maximal inhibitory concentration (IC₅₀) values are graphically obtained from the dose-response curves^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chambers JM, et al. Synthesis of biotinylated episilvestrol: highly selective targeting of the translation factors eIF4AI/II. Org Lett. 2013 Mar 15;15(6):1406-9. [Content Brief]

[2]. Hwang BY, et al. Silvestrol and episilvestrol, potential anticancer rocaglate derivatives from Aglaia silvestris. J Org Chem. 2004 May 14;69(10):3350-8. [Content Brief]

[3]. Daker M, et al. Inhibition of nasopharyngeal carcinoma cell proliferation and synergism of cisplatin with silvestrol and episilvestrol isolated from Aglaia stellatopilosa. Exp Ther Med. 2016 Jun;11(6):2117-2126. Epub 2016 Mar 29. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5275 mL	7.6376 mL	15.2751 mL	38.1878 mL
	5 mM	0.3055 mL	1.5275 mL	3.0550 mL	7.6376 mL
	10 mM	0.1528 mL	0.7638 mL	1.5275 mL	3.8188 mL
	15 mM	0.1018 mL	0.5092 mL	1.0183 mL	2.5459 mL
	20 mM	0.0764 mL	0.3819 mL	0.7638 mL	1.9094 mL
	25 mM	0.0611 mL	0.3055 mL	0.6110 mL	1.5275 mL
	30 mM	0.0509 mL	0.2546 mL	0.5092 mL	1.2729 mL

Episilvestrol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Episilvestrol697235-39-5Eukaryotic Initiation Factor (eIF)AutophagyInhibitorinhibitorinhibit

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Episilvestrol

Other Forms of Episilvestrol:

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