2. Neuronal Signaling
  3. α-synuclein
  4. Fast Green FCF

Fast Green FCF  (Synonyms: FD&C Green No. 3; Food green 3; C.I. 42053)

Cat. No.: HY-D0914 Purity: 99.95%
SDS
COA
Handling Instructions Technical Support

Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function.

For research use only. We do not sell to patients.

Fast Green FCF Chemical Structure

Fast Green FCF Chemical Structure

CAS No. : 2353-45-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Fast Green FCF:

Fast Green FCF Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function[1][2][3][4][5][6][7][8][9].

IC50 & Target[1][2][3][4][5][6][7][8][9]

α-synuclein Aggregation

 

In Vitro

Fast Green FCF (molar ratios of HEWL/FCF: 1 to 0.01-1 to 1, 10 days) suppresses the generation of lysozyme fibrils in a concentration-dependent manner and exhibits disaggregating activity against the preformed/existing lysozyme fibrils[2].
Fast Green FCF (100 μg/mL-1 mg/mL) inhibits synaptic activity in rat hippocampal interneurons, with half-maximum effective concentration of ~200 μg/mL (250 μM)[3].
Fast Green FCF (5-100 μM, 24 h) inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity in PC12 cells[4].
Fast green FCF (molar ratios of Aβ40 to FGF of 3:1-1:3) alleviates the cytotoxicity induced by Aβ40 fibrillogenesis in PC12 cells[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fast Green FCF Related Antibodies

Cell Viability Assay[4]

Cell Line: PC12 cells with α-syn fibrils
Concentration: 5, 25, 50, 100 μM
Incubation Time: 24 h
Result: Reduced the cytotoxicity, and the cell viability respectively reached 66.2% (5 μM) and 72.7% (25 μM).
Increased cell viability, reaching 76.4% (50 μM) and 89.0% (100 μM), respectively.
In Vivo

Fast Green FCF (100 mg/kg, i.p., 30 min prior to each trial) attenuates Aβ-induced cognitive impairment in mice[5]. Fast Green FCF (10-100 mg/kg, i.p., once daily, 30 min before surgery) prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice[6]. Fast Green FCF (100 mg/kg, i.p., daily for 7 days) attenuates lipopolysaccharide-induced depressive-like behavior and downregulates TLR4/Myd88/NF-κB signal pathway in the mouse hippocampus[7]. Fast Green FCF (30-100 mg/kg, i.p., daily for 9 days) alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model[8]. Fast Green FCF (0.017-0.041 gm per kg body weight, p.o., 42 days) causes a significant decrease in testicular weight, tubular diameter, sperm density, and sperm motility[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Aβ-induced cognitive impairment mice[5]
Dosage: 30, 100 mg/kg
Administration: Intraperitoneal injection (i.p.), 30 min prior to each trial
Result: Did not alter the recognition index.
Prevented the Aβ42-induced recognition impairment at 100 mg/kg.
Prevented the Aβ42-induced impairment of spatial learning at 100 mg/kg.
reduced the Aβ42-induced memory impairment.
Molecular Weight

808.85

Formula

C37H34N2Na2O10S3
CAS No.
Appearance

Solid

Color

Dark green to black

Emission (Em)

522
Excitation (Ex)

486
SMILES

O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (12.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2363 mL 6.1816 mL 12.3632 mL
5 mM 0.2473 mL 1.2363 mL 2.4726 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 4.55 mg/mL (5.63 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.95%

SDS (584 KB)

COA (259 KB) HNMR (325 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2363 mL 6.1816 mL 12.3632 mL 30.9081 mL
5 mM 0.2473 mL 1.2363 mL 2.4726 mL 6.1816 mL
10 mM 0.1236 mL 0.6182 mL 1.2363 mL 3.0908 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Fast Green FCF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fast Green FCF2353-45-9FD&C Green No. 3 Food green 3 C.I. 42053Food green3Food green 3Food green-3α-synucleinα-Synprotein stainquantitative stainhistonescarcinogenicInhibitorinhibitorinhibit

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