Fast green FCF free acid 

Fast green FCF free acid is a dye that is acid-resistant. Fast Green FCF free acid inhibits α-synuclein, Aβ, P2X4 receptor and TLR4/Myd88/NF-κB. Fast Green FCF free acid is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF free acid improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function^{[1][2][3][4][5][6][7][8][9]}.

Fast Green FCF free acid (molar ratios of HEWL/FCF free acid: 1 to 0.01-1 to 1, 10 days) free acid suppresses the generation of lysozyme fibrils in a concentration-dependent manner and exhibits disaggregating activity against the preformed/existing lysozyme fibrils^[2].
Fast Green FCF (100 μg/mL-1 mg/mL) free acid inhibits synaptic activity in rat hippocampal interneurons, with half-maximum effective concentration of ~200 μg/mL (250 μM)^[3].
Fast Green FCF (5-100 μM, 24 h) free acid inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity in PC12 cells^[4].
Fast green FCF (molar ratios of Aβ40 to FGF of 3:1-1:3) free acid alleviates the cytotoxicity induced by Aβ40 fibrillogenesis in PC12 cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fast Green FCF (100 mg/kg, i.p., 30 min prior to each trial) free acid attenuates Aβ-induced cognitive impairment in mice^[5]. Fast Green FCF (10-100 mg/kg, i.p., once daily, 30 min before surgery) free acid prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice^[6]. Fast Green FCF (100 mg/kg, i.p., daily for 7 days) free acid attenuates lipopolysaccharide-induced depressive-like behavior and downregulates TLR4/Myd88/NF-κB signal pathway in the mouse hippocampus^[7]. Fast Green FCF (30-100 mg/kg, i.p., daily for 9 days) free acid alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model^[8]. Fast Green FCF (0.017-0.041 gm per kg body weight, p.o., 42 days) free acid causes a significant decrease in testicular weight, tubular diameter, sperm density, and sperm motility^[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

764.88

C₃₇H₃₆N₂O₁₀S₃

25738-40-3

O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O)=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O)=O)=CC=C1)([O-])=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Murakami T, et al.Establishment of novel meniscal scaffold structures using polyglycolic and poly-l-lactic acids. J Biomater Appl. 2017 Aug;32(2):150-161.