Fluorescein Biotin

Cat. No.: HY-D1030 Purity: 97.27%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Fluorescein Biotin is used as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance; the the fluorescence or absorbance of Fluorescein Biotin is quenched, upon binding to avidin or streptavidin.

For research use only. We do not sell to patients.

Fluorescein Biotin Chemical Structure

CAS No. : 134759-22-1

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

831.01

Formula

C₄₂H₅₀N₆O₈S₂

CAS No.

134759-22-1

Appearance

Solid

Color

Yellow to orange

SMILES

O=C1OC2(C3=C(C=C(O)C=C3)OC4=CC(O)=CC=C24)C5=C1C=C(NC(NCCCCCNC(CCCCCNC(CCCC[C@H]6[C@](N7)([H])[C@](NC7=O)([H])CS6)=O)=O)=S)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (60.17 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2034 mL	6.0168 mL	12.0335 mL
5 mM	0.2407 mL	1.2034 mL	2.4067 mL
10 mM	0.1203 mL	0.6017 mL	1.2034 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.27%

Select Batch:

Data Sheet (266 KB) SDS (252 KB)

COA (243 KB) RP-HPLC (254 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Gruber HJ, et al. Accurate titration of avidin and streptavidin with biotin-fluorophore conjugates in complex, colored biofluids. Biochim Biophys Acta. 1998 Jul 23;1381(2):203-12. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2034 mL	6.0168 mL	12.0335 mL	30.0839 mL
	5 mM	0.2407 mL	1.2034 mL	2.4067 mL	6.0168 mL
	10 mM	0.1203 mL	0.6017 mL	1.2034 mL	3.0084 mL
	15 mM	0.0802 mL	0.4011 mL	0.8022 mL	2.0056 mL
	20 mM	0.0602 mL	0.3008 mL	0.6017 mL	1.5042 mL
	25 mM	0.0481 mL	0.2407 mL	0.4813 mL	1.2034 mL
	30 mM	0.0401 mL	0.2006 mL	0.4011 mL	1.0028 mL
	40 mM	0.0301 mL	0.1504 mL	0.3008 mL	0.7521 mL
	50 mM	0.0241 mL	0.1203 mL	0.2407 mL	0.6017 mL
	60 mM	0.0201 mL	0.1003 mL	0.2006 mL	0.5014 mL