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  3. Fluorescent Dye
  4. Hoechst 33342 analog 2

Hoechst 33342 analog 2 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

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Hoechst 33342 analog 2 Chemical Structure

Hoechst 33342 analog 2 Chemical Structure

CAS No. : 106050-84-4

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Hoechst 33342 analog 2 Related Antibodies

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Description

Hoechst 33342 analog 2 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

In Vitro

General Protocol
Preparation of Hoechst working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of in 5 mL DMSO
Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
1.Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Precautions
1. Please adjust the concentration of Hoechst working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hoechst 33342 analog 2 Related Antibodies
Molecular Weight

550.39

Formula

C25H23IN6O
CAS No.
Appearance

Solid

Color

Pale purple to khaki

Emission (Em)

451
Excitation (Ex)

356
SMILES

OC1=CC=C(C2=NC3=CC(C4=NC5=CC(N6CCN(C)CC6)=CC=C5N4)=CC=C3N2)C=C1I
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 57 mg/mL (103.56 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8169 mL 9.0845 mL 18.1689 mL
5 mM 0.3634 mL 1.8169 mL 3.6338 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8169 mL 9.0845 mL 18.1689 mL 45.4223 mL
5 mM 0.3634 mL 1.8169 mL 3.6338 mL 9.0845 mL
10 mM 0.1817 mL 0.9084 mL 1.8169 mL 4.5422 mL
15 mM 0.1211 mL 0.6056 mL 1.2113 mL 3.0282 mL
20 mM 0.0908 mL 0.4542 mL 0.9084 mL 2.2711 mL
25 mM 0.0727 mL 0.3634 mL 0.7268 mL 1.8169 mL
30 mM 0.0606 mL 0.3028 mL 0.6056 mL 1.5141 mL
40 mM 0.0454 mL 0.2271 mL 0.4542 mL 1.1356 mL
50 mM 0.0363 mL 0.1817 mL 0.3634 mL 0.9084 mL
60 mM 0.0303 mL 0.1514 mL 0.3028 mL 0.7570 mL
80 mM 0.0227 mL 0.1136 mL 0.2271 mL 0.5678 mL
100 mM 0.0182 mL 0.0908 mL 0.1817 mL 0.4542 mL
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Hoechst 33342 analog 2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hoechst 33342 analog 2106050-84-4Fluorescent DyeHoechstDNAcellular dyeblue fluorescenceInhibitorinhibitorinhibit

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