2. Neuronal Signaling
  3. Amyloid-β
  4. Hoechst 34580

Hoechst 34580 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

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Hoechst 34580 Chemical Structure

Hoechst 34580 Chemical Structure

CAS No. : 23555-00-2

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Hoechst 34580 Related Antibodies

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Description

Hoechst 34580 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

IC50 & Target

Amyloid-β[1]
In Vitro

General Protocol
Preparation of Hoechst working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of in 5 mL DMSO
Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
1.Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Precautions
1. Please adjust the concentration of Hoechst working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hoechst 34580 Related Antibodies
Molecular Weight

451.57

Formula

C27H29N7
CAS No.
Appearance

Solid

Color

Off-white to pink

Emission (Em)

451
Excitation (Ex)

356
SMILES

CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(N(C)C)C=C6)N5)=C4)N3)=C2)CC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 47 mg/mL (104.08 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2145 mL 11.0725 mL 22.1450 mL
5 mM 0.4429 mL 2.2145 mL 4.4290 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2145 mL 11.0725 mL 22.1450 mL 55.3624 mL
5 mM 0.4429 mL 2.2145 mL 4.4290 mL 11.0725 mL
10 mM 0.2214 mL 1.1072 mL 2.2145 mL 5.5362 mL
15 mM 0.1476 mL 0.7382 mL 1.4763 mL 3.6908 mL
20 mM 0.1107 mL 0.5536 mL 1.1072 mL 2.7681 mL
25 mM 0.0886 mL 0.4429 mL 0.8858 mL 2.2145 mL
30 mM 0.0738 mL 0.3691 mL 0.7382 mL 1.8454 mL
40 mM 0.0554 mL 0.2768 mL 0.5536 mL 1.3841 mL
50 mM 0.0443 mL 0.2214 mL 0.4429 mL 1.1072 mL
60 mM 0.0369 mL 0.1845 mL 0.3691 mL 0.9227 mL
80 mM 0.0277 mL 0.1384 mL 0.2768 mL 0.6920 mL
100 mM 0.0221 mL 0.1107 mL 0.2214 mL 0.5536 mL
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Hoechst 34580 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hoechst 3458023555-00-2HOE 34580Hoechst34580Hoechst-34580HOE34580HOE-34580Amyloid-ββ-amyloid peptideAβAbetaHoechstDNAcellular dyeblue fluorescenceInhibitorinhibitorinhibit

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