IZCZ-3

Name: IZCZ-3
Brand: MedChemExpress
SKU: HY-111411
Rating: 5.0 (3 reviews)

Cat. No.: HY-111411 Purity: 98.13%: FAQs
Data Sheet SDS COA Handling Instructions

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IZCZ-3 is a potent c-MYC transcription inhibitor with antitumor activity.

For research use only. We do not sell to patients.

IZCZ-3 Chemical Structure

CAS No. : 2223019-53-0

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5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

2 Publications Citing Use of MCE IZCZ-3

: IZCZ-3 purchased from MedChemExpress. Usage Cited in: Oncogene. 2022 Oct 21. [Abstract]; IZCZ-3 (0.5 μM; 24 h) co-operates with IFNγ to induce differentiation in NB4 cells.

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Description

IZCZ-3 is a potent c-MYC transcription inhibitor with antitumor activity^[1].

IC₅₀ & Target

c-MYC transcription^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	4.2 μM Compound: IZCZ-3	Cytotoxicity against human A375 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human A375 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29474069]
BJ	IC₅₀	15.9 μM Compound: IZCZ-3	Cytotoxicity against human BJ cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human BJ cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29474069]
HBL-100	IC₅₀	11.2 μM Compound: 1; IZCZ-3	Cytotoxicity against human HBL-100 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HBL-100 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 36121464]
HeLa	IC₅₀	2.1 μM Compound: IZCZ-3	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29474069]
HepG2	IC₅₀	6.1 μM Compound: 1; IZCZ-3	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 36121464]
Huh-7	IC₅₀	4.1 μM Compound: IZCZ-3	Cytotoxicity against human Huh7 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human Huh7 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29474069]
MDA-MB-231	IC₅₀	5.1 μM Compound: 1; IZCZ-3	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 36121464]
SiHa	IC₅₀	3.3 μM Compound: IZCZ-3	Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human SiHa cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29474069]
U2OS	IC₅₀	6.8 μM Compound: IZCZ-3	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29474069]

In Vitro

IZCZ-3 (2.1 μM-15.9 μM; 24 hours) significantly inhibits SiHa, HeLa, Huh7, and A375 cancer cell proliferation (IC₅₀s of 3.3, 2.1，4.1, and 4.2 μM, respectively). IZCZ-3 induces only weak growth inhibition in the BJ fibroblasts (IC₅₀=15.9 μM) and mouse mesangial cells (IC₅₀=15.6 μM), suggesting that IZCZ-3 is more effective against cancer cells than against c-MYC-independent normal cells^[1].
IZCZ-3 (0-5 μM; 12 hours) induces an apparent accumulation of cells in the G0/G1 phase in SiHa cells in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	SiHa, HeLa, Huh7, and A375 cancer cells (with overexpression of c-MYC protein) and in normal BJ fibroblasts and primary cultured mouse mesangial cells (with relatively low expression of c-MYC protein).
Concentration:	2.1 μM-15.9 μM
Incubation Time:	24 hours
Result:	IC₅₀s of 3.3, 2.1，4.1, and 4.2 μM for SiHa, HeLa, Huh7, and A375 cancer cells; IC₅₀s of 15.9 μM and 15.6 μM for BJ fibroblasts and mouse mesangial cells.

Cell Cycle Analysis^[1]

Cell Line:	SiHa cells
Concentration:	0, 1.25, 2.5, and 5 μM
Incubation Time:	12 hours
Result:	Induced an apparent accumulation of cells in the G0/G1 phase (increasing from 61% to 70%) in a dose-dependent manner.

In Vivo

IZCZ-3 (20, 10, and 5 mg/kg; intraperitoneally; every other day for 24 days) inhibits tumor growth in BALB/c nude mice with SiHa human cervical squamous cancer xenograft^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (5 weeks old) bearing SiHa human cervical squamous cancer xenograft model^[1]
Dosage:	20, 10, and 5 mg/kg
Administration:	Treated intraperitoneally; every other day for 24 days
Result:	Treatment with 20, 10, and 5 mg/kg resulted in a significant reduction in tumor weight with tumor growth inhibition (TGI) of 69%, 64%, and 57%, respectively. Displayed time-dependent inhibition of tumor growth.

Molecular Weight

715.93

Formula

C₄₆H₄₉N₇O

CAS No.

2223019-53-0

Appearance

Solid

Color

Off-white to pink

SMILES

CN(CC1)CCN1C2=CC=C(C3=C(C4=CC=C(N5CCN(C)CC5)C=C4)N=C(C6=CC(C(C=CC=C7)=C7N8CC)=C8C=C6)N3C9=CC=C(OC)C=C9)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (6.98 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3968 mL	6.9839 mL	13.9678 mL
5 mM	0.2794 mL	1.3968 mL	2.7936 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.13%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (257 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Hu MH, et al. Discovery of a New Four-Leaf Clover-Like Ligand as a Potent c-MYC Transcription Inhibitor Specifically Targeting the Promoter G-Quadruplex. J Med Chem. 2018 Mar 22;61(6):2447-2459. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3968 mL	6.9839 mL	13.9678 mL	34.9196 mL
DMSO	5 mM	0.2794 mL	1.3968 mL	2.7936 mL	6.9839 mL

IZCZ-3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IZCZ-32223019-53-0IZCZ3IZCZ 3c-MycAutophagyMycInhibitorinhibitorinhibit

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IZCZ-3

Customer Review

IZCZ-3 Related Antibodies

2 Publications Citing Use of MCE IZCZ-3

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Complete Stock Solution Preparation Table

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