1. Metabolic Enzyme/Protease Apoptosis Protein Tyrosine Kinase/RTK NF-κB Immunology/Inflammation
  2. Endogenous Metabolite Ferroptosis ROS Kinase Keap1-Nrf2 Reactive Oxygen Species
  3. L-Cystine

L-Cystine is an orally active extracellular form of L-Cysteine (HY-Y0337), occurring in proteins of plants and animals. L-Cystine elevates Nrf2 protein expression and activates Nrf2 transcription factor. L-cystine reduces ROS generation and protects against oxidant- or Doxorubicin (HY-15142A)-induced apoptosis. L-Cystine combined with L-theanine (HY-15121) enhances the production of antigen-specific IgG by increasing glutathione (GSH) levels and T helper 2 (Th2) mediated responses in mice. L-Cystine is promising for research of cystinuria and kidney stones

For research use only. We do not sell to patients.

L-Cystine Chemical Structure

L-Cystine Chemical Structure

CAS No. : 56-89-3

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500 mg In-stock
1 g In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of L-Cystine:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

L-Cystine is an orally active extracellular form of L-Cysteine (HY-Y0337), occurring in proteins of plants and animals. L-Cystine elevates Nrf2 protein expression and activates Nrf2 transcription factor. L-cystine reduces ROS generation and protects against oxidant- or Doxorubicin (HY-15142A)-induced apoptosis. L-Cystine combined with L-theanine (HY-15121) enhances the production of antigen-specific IgG by increasing glutathione (GSH) levels and T helper 2 (Th2) mediated responses in mice. L-Cystine is promising for research of cystinuria and kidney stones[1][2][3][4][5][6]

IC50 & Target

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
KB 3-1 IC50
3.12 mM
Compound: cystine
Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
[PMID: 21657271]
KB-V1 IC50
2.14 mM
Compound: cystine
Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
[PMID: 21657271]
In Vitro

L-Cystine (83 µM or 200 µM, 24 h or 72 h) effectively reverses the growth inhibition of both Sulfasalazine (HY-14655) and Erastin (HY-15763) in Huh6 cells[1].
L-Cystine ( 0.8 mM, 4 h) induces Nrf2 protein expression elevation in a Keap1-dependent manner in HeLa cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Huh6 and Huh7 cells
Concentration: 83 µM or 200 µM
Incubation Time: 72 h or 24 h
Result: The degree of growth inhibition by Sulfasalazine (HY-14655) or Erastin (HY-15763) was greater in medium containing a physiological level of 83 μM L-cystine than in medium containing 200 μM L-cystine in Huh6 and Huh7 cells.

Western Blot Analysis[6]

Cell Line: HeLa cells
Concentration: 0.2 mM
Incubation Time: 4 h
Result: Was sufficient for producing Nrf2 protein elevation at 0.2 mM in HeLa cells.

Western Blot Analysis[6]

Cell Line: HeLa cells
Concentration: 0.8 mM
Incubation Time: 4 h
Result: Induced Nrf2 in a Keap1-dependent manner in HeLa cells.
In Vivo

L-Cystine (280 mg/kg, p.o., 11 days) administrated with L-theanine (HY-15121) enhances antigen-specific IgG production partly through augmentation of glutathione (GSH) levels and T helper 2 (Th2)-mediated responses in mice[2].
L-Cystine (200 mg/kg, p.o., 11 days) administrated with L-theanine (HY-15121) (80 mg/kg, p.o., 11 days) significantly decreases the serum IL-10/IFN-γ ratio 6 hours after DNP-KLH antigenic stimulation, but increases the serum IL-10/IFN-γ ratio 24 hours after DNP-KLH antigenic stimulation in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nine-week-old female BALB/c mice[2]
Dosage: 140, 280 and 560 mg/kg
Administration: p.o. administration for 11 days
Result: Significantly increased IgG and IgM antibody production administrated with L-theanine (HY-15121) after DNP-dextran and DNP-KLH antigenic stimulation in mice.
Molecular Weight

240.30

Formula

C6H12N2O4S2

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CSSC[C@@H](C(O)=O)N)C(O)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 33.33 mg/mL (138.70 mM; ultrasonic and adjust pH to 11 with 1 M NaOH)

0.1 M NaOH : 3.33 mg/mL (13.86 mM; ultrasonic and warming and adjust pH to 11 with NaOH and heat to 70°C)

0.1 M HCL : 2.5 mg/mL (10.40 mM; ultrasonic and adjust pH to 2 with HCl)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1615 mL 20.8073 mL 41.6146 mL
5 mM 0.8323 mL 4.1615 mL 8.3229 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.84%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
0.1 M HCL / 0.1 M NaOH / H2O 1 mM 4.1615 mL 20.8073 mL 41.6146 mL 104.0366 mL
5 mM 0.8323 mL 4.1615 mL 8.3229 mL 20.8073 mL
10 mM 0.4161 mL 2.0807 mL 4.1615 mL 10.4037 mL
H2O 15 mM 0.2774 mL 1.3872 mL 2.7743 mL 6.9358 mL
20 mM 0.2081 mL 1.0404 mL 2.0807 mL 5.2018 mL
25 mM 0.1665 mL 0.8323 mL 1.6646 mL 4.1615 mL
30 mM 0.1387 mL 0.6936 mL 1.3872 mL 3.4679 mL
40 mM 0.1040 mL 0.5202 mL 1.0404 mL 2.6009 mL
50 mM 0.0832 mL 0.4161 mL 0.8323 mL 2.0807 mL
60 mM 0.0694 mL 0.3468 mL 0.6936 mL 1.7339 mL
80 mM 0.0520 mL 0.2601 mL 0.5202 mL 1.3005 mL
100 mM 0.0416 mL 0.2081 mL 0.4161 mL 1.0404 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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L-Cystine
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HY-N0394
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