2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. MCLA hydrochloride

MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.

For research use only. We do not sell to patients.

MCLA hydrochloride Chemical Structure

MCLA hydrochloride Chemical Structure

CAS No. : 128322-44-1

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Based on 1 publication(s) in Google Scholar

MCLA hydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE MCLA hydrochloride

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  • Purity & Documentation

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  • Customer Review

Description

MCLA hydrochloride is a chemiluminescent reagent which can be used to quantify aqueous concentrations of superoxide.

In Vitro

The non-specific luminescence remains almost constant for 10 min after the addition of MCLA hydrochloride (MCLA) and is not significantly influenced by SOD. The MCLA hydrochloride method is, however, 4.5-times more sensitive than the CLA method[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MCLA hydrochloride Related Antibodies
Molecular Weight

291.73

Formula

C14H14ClN3O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=NC2=CNC(C3=CC=C(OC)C=C3)=CN2C1=O.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (34.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4278 mL 17.1391 mL 34.2783 mL
5 mM 0.6856 mL 3.4278 mL 6.8557 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.81%

SDS (393 KB)

COA (249 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4278 mL 17.1391 mL 34.2783 mL 85.6957 mL
5 mM 0.6856 mL 3.4278 mL 6.8557 mL 17.1391 mL
10 mM 0.3428 mL 1.7139 mL 3.4278 mL 8.5696 mL
15 mM 0.2285 mL 1.1426 mL 2.2852 mL 5.7130 mL
20 mM 0.1714 mL 0.8570 mL 1.7139 mL 4.2848 mL
25 mM 0.1371 mL 0.6856 mL 1.3711 mL 3.4278 mL
30 mM 0.1143 mL 0.5713 mL 1.1426 mL 2.8565 mL
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MCLA hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MCLA128322-44-1Reactive Oxygen SpeciesInhibitorinhibitorinhibit

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