ML204 hydrochloride

Name: ML204 hydrochloride
Brand: MedChemExpress
SKU: HY-12949A
Rating: 5.0 (5 reviews)

Cat. No.: HY-12949A Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

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ML204 hydrochloride is a novel, potent, selective TRPC4/TRPC5 channel inhibitor, with at least 19-fold selectivity against TRPC6 and no appreciable effect on all other TRP channels, nor on voltage-gated sodium, potassium, or Ca²⁺ channels.

For research use only. We do not sell to patients.

ML204 hydrochloride Chemical Structure

CAS No. : 2070015-10-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of ML204 hydrochloride:

ML204 In-stock

5 Publications Citing Use of MCE ML204 hydrochloride

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TRPC TRPM TRPV TRPA TRPML

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

TRPC4

TRPC5

In Vitro

ML204 hydrochloride inhibits TRPC4β-mediated intracellular Ca²⁺ rise with an IC₅₀ value of 0.96 μM (HEK293 cells) and exhibits 19-fold selectivity against muscarinic receptor-coupled TRPC6 channel activation^[1].
ML204 hydrochloride blocks TRPC4β activity induced through either G_i/o stimulation by μ-opioid, 5HT_1A serotonin, and M₂ muscarinic receptors or G_q/11 stimulation by the endogenous M₃-like muscarinic receptors^[1].
ML204 hydrochloride blocks LPS-induced TRPC5 channel activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ML204 hydrochloride (1 mg/kg; s.c.; twice a day; for 5 days) causes mortality associated with exacerbated hypothermia and decreases peritoneal leukocyte numbers and cytokines in LPS-injected mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nonfasted male C57BL/6 (2-3 months)^[4]
Dosage:	1 mg/kg
Administration:	Subcutaneous injection, twice a day, for 5 days (prior to LPS injection)
Result:	Induces mortality associated with increased hypothermia in mice with LPS-induced systemic inflammatory response.

Molecular Weight

262.78

Formula

C₁₅H₁₉ClN₂

CAS No.

2070015-10-8

Appearance

Solid

Color

White to off-white

SMILES

CC1=CC(N2CCCCC2)=NC3=CC=CC=C13.Cl[H]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 30 mg/mL (114.16 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8055 mL	19.0273 mL	38.0546 mL
5 mM	0.7611 mL	3.8055 mL	7.6109 mL
10 mM	0.3805 mL	1.9027 mL	3.8055 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (250 KB) HNMR (260 KB) LCMS (86 KB)

Handling Instructions (2659 KB)

References

[1]. Miller M, et al. Identification of ML204, a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels. J Biol Chem. 2011 Sep 23;286(38):33436-46. [Content Brief]

[2]. Miller MR, et al. Novel Chemical Inhibitor of TRPC4 Channels. Probe Reports from the NIH Molecular Libraries Program. [Content Brief]

[3]. Thomas Schaldecker, et al. Inhibition of the TRPC5 ion channel protects the kidney filter. J Clin Invest. 2013 Dec 2; 123(12): 5298–5309. [Content Brief]

[4]. Domingos M S Pereira, et al. Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice. Oxid Med Cell Longev. 2018 Jun 10;2018:4904696. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.8055 mL	19.0273 mL	38.0546 mL	95.1366 mL
	5 mM	0.7611 mL	3.8055 mL	7.6109 mL	19.0273 mL
	10 mM	0.3805 mL	1.9027 mL	3.8055 mL	9.5137 mL
	15 mM	0.2537 mL	1.2685 mL	2.5370 mL	6.3424 mL
	20 mM	0.1903 mL	0.9514 mL	1.9027 mL	4.7568 mL
	25 mM	0.1522 mL	0.7611 mL	1.5222 mL	3.8055 mL
	30 mM	0.1268 mL	0.6342 mL	1.2685 mL	3.1712 mL
	40 mM	0.0951 mL	0.4757 mL	0.9514 mL	2.3784 mL
	50 mM	0.0761 mL	0.3805 mL	0.7611 mL	1.9027 mL
	60 mM	0.0634 mL	0.3171 mL	0.6342 mL	1.5856 mL
	80 mM	0.0476 mL	0.2378 mL	0.4757 mL	1.1892 mL
	100 mM	0.0381 mL	0.1903 mL	0.3805 mL	0.9514 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.