2. Membrane Transporter/Ion Channel
  3. Potassium Channel
  4. ML277

ML277 (CID-53347902) is a potent and selective activator of K(v)7.1 (KCNQ1) potassium channel activator (EC50=270 nM), rescues function of pathophysiologically important mutant channel complexes in human induced pluripotent stem cell-derived cardiomyocytes.

For research use only. We do not sell to patients.

ML277 Chemical Structure

ML277 Chemical Structure

CAS No. : 1401242-74-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 100 In-stock
Solution
10 mM * 1 mL in DMSO USD 100 In-stock
Solid
1 mg USD 38 In-stock
5 mg USD 96 In-stock
10 mg USD 144 In-stock
25 mg USD 260 In-stock
50 mg USD 390 In-stock
100 mg USD 585 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

ML277 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

ML277 (CID-53347902) is a potent and selective activator of K(v)7.1 (KCNQ1) potassium channel activator (EC50=270 nM), rescues function of pathophysiologically important mutant channel complexes in human induced pluripotent stem cell-derived cardiomyocytes[1][2].

In Vitro

ML277 (1 μM) increases the amplitude of KCNQ1 whole-cell and single-channel currents[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ML277 Related Antibodies
Molecular Weight

471.59

Formula

C23H25N3O4S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H]1N(S(=O)(C2=CC=C(C)C=C2)=O)CCCC1)NC3=NC(C4=CC=C(OC)C=C4)=CS3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 56 mg/mL (118.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1205 mL 10.6024 mL 21.2049 mL
5 mM 0.4241 mL 2.1205 mL 4.2410 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1205 mL 10.6024 mL 21.2049 mL 53.0122 mL
5 mM 0.4241 mL 2.1205 mL 4.2410 mL 10.6024 mL
10 mM 0.2120 mL 1.0602 mL 2.1205 mL 5.3012 mL
15 mM 0.1414 mL 0.7068 mL 1.4137 mL 3.5341 mL
20 mM 0.1060 mL 0.5301 mL 1.0602 mL 2.6506 mL
25 mM 0.0848 mL 0.4241 mL 0.8482 mL 2.1205 mL
30 mM 0.0707 mL 0.3534 mL 0.7068 mL 1.7671 mL
40 mM 0.0530 mL 0.2651 mL 0.5301 mL 1.3253 mL
50 mM 0.0424 mL 0.2120 mL 0.4241 mL 1.0602 mL
60 mM 0.0353 mL 0.1767 mL 0.3534 mL 0.8835 mL
80 mM 0.0265 mL 0.1325 mL 0.2651 mL 0.6627 mL
100 mM 0.0212 mL 0.1060 mL 0.2120 mL 0.5301 mL
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ML277 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML2771401242-74-7CID-53347902ML 277ML-277CID53347902CID 53347902Potassium ChannelKcsAhuman induced pluripotent stem cellcardiomyocyteschannel complexeInhibitorinhibitorinhibit

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