2. GPCR/G Protein Neuronal Signaling
  3. Neurotensin Receptor
  4. Neurotensin(8-13)

Neurotensin (8-13) is an active fragment of Neurotensin. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Neurotensin(8-13) Chemical Structure

Neurotensin(8-13) Chemical Structure

CAS No. : 60482-95-3

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5 mg USD 90 In-stock
10 mg USD 156 In-stock
25 mg USD 348 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Neurotensin(8-13):

Neurotensin(8-13) Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Neurotensin(8-13)

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NTR1 NTR2

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Neurotensin (8-13) is an active fragment of Neurotensin. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.

IC50 & Target

NTR1

 

In Vitro

Receptor internalization induced by Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density. The receptor downregulation in response to high extracellular concentrations of the peptide has been described for Neurotensin (NT) in HT-29 cells and in rat primary cultured neurons. Reappearance of the receptors on the cell surface is also different[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Neurotensin(8-13) Related Antibodies
Molecular Weight

816.99

Formula

C38H64N12O8
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Arg-Arg-Pro-Tyr-Ile-Leu
Sequence Shortening

RRPYIL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (61.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2240 mL 6.1200 mL 12.2401 mL
5 mM 0.2448 mL 1.2240 mL 2.4480 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 33.33 mg/mL (40.80 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Kinase Assay
[1]

Binding assays are performed on whole HT-29 cells at confluence. A day before the assay, cells (106 cells/0.4 mL, equivalent to 0.3 mg protein) are placed in 48-well plates. A special binding buffer that includes protease inhibitors (50 mM HEPES, 125 mM NaCl, 7.5 mM KCl, 5.5 mM MgCl2, 1 mM EGTA, 5 g/L bovine serum albumin, 2 mg/L chymostatin, 100 mg/L soybean trypsin inhibitor, 50 mg/L bacitracin, pH 7.4) is used for the experiments. In inhibition studies, cells are incubated for 1 h at 37°C in triplicate with 25,000 cpm of 125I-NT and variable concentrations (0.001-3,000 nM) of unlabeled NT(8-13), unlabeled NT-VIII, or NT-VIII labeled with natRe (final volume of 0.2 mL per well). The cells are then washed twice with cold binding buffer and afterward are solubilized with 1N NaOH at 37°C (0.4 mL per well). The activity is determined in a γ-counter. In saturation studies, cells are incubated in triplicate with increasing concentrations (0.1-10 nM) of 99mTc(CO)3NT-VIII for 1 h at 37°C (final volume, 0.2 mL per well). The concentrations of total technetium (99+99mTc) are equivalent to 0.2-20 MBq 99mTc activity per well. After 2 washings with the same binding buffer as before, the cells are then solubilized with 1N NaOH at 37°C (0.4 mL per well). The bound radioactivity is measured in the γ-counter. Nonspecific binding is determined with 1 μM unlabeled NT(8-13)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2240 mL 6.1200 mL 12.2401 mL 30.6001 mL
5 mM 0.2448 mL 1.2240 mL 2.4480 mL 6.1200 mL
10 mM 0.1224 mL 0.6120 mL 1.2240 mL 3.0600 mL
15 mM 0.0816 mL 0.4080 mL 0.8160 mL 2.0400 mL
20 mM 0.0612 mL 0.3060 mL 0.6120 mL 1.5300 mL
25 mM 0.0490 mL 0.2448 mL 0.4896 mL 1.2240 mL
30 mM 0.0408 mL 0.2040 mL 0.4080 mL 1.0200 mL
40 mM 0.0306 mL 0.1530 mL 0.3060 mL 0.7650 mL
50 mM 0.0245 mL 0.1224 mL 0.2448 mL 0.6120 mL
60 mM 0.0204 mL 0.1020 mL 0.2040 mL 0.5100 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Neurotensin(8-13) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neurotensin(8-13)60482-95-3Neurotensin ReceptorInhibitorinhibitorinhibit

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