2. Others Metabolic Enzyme/Protease Apoptosis
  3. Fluorescent Dye Glutathione Peroxidase
  4. Phenol Red sodium salt

Phenol Red sodium salt  (Synonyms: Phenolsulfonephthalein sodium salt)

Cat. No.: HY-D0169A Purity: ≥97.0%
SDS
COA
Handling Instructions Technical Support

Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye. Phenol Red sodium salt is also an antagonist of rat P2X1R. Phenol Red sodium salt enhances the halogenating activity of myeloperoxidase. Phenol Red sodium salt shows a distinct color change from pink to yellow in a positive pH reaction.

For research use only. We do not sell to patients.

Phenol Red sodium salt Chemical Structure

Phenol Red sodium salt Chemical Structure

CAS No. : 34487-61-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 g In-stock
25 g In-stock
50 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Phenol Red sodium salt:

Phenol Red sodium salt Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Phenol Red sodium salt

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye. Phenol Red sodium salt is also an antagonist of rat P2X1R. Phenol Red sodium salt enhances the halogenating activity of myeloperoxidase. Phenol Red sodium salt shows a distinct color change from pink to yellow in a positive pH reaction[1][2][3][4].

In Vitro

Phenol Red sodium salt acts as an indicator of AChE activity[4]
1.Solutions Preparation
Concentration of Phenol Red sodium salt in water is set to 500 µM.
2. Reaction
1) Thirty-five microliters of PBS, 25 µL of PBS 7.4 or tacrine solution, 20 µL of Phenol Red sodium salt, and 20 µL of 5 mM AChCl are consequently added to the surface of paper with immobilized AChE.
2) After 5 min of incubation, the surface of the paper is photographed using a smart phone camera placed on a 3D-printed, tube-shaped holder.
Note: in neutral pH of PBS 7.4, Phenol red gives a red color. After addition of AChCl, immediately forming acetic acid acidifying medium and the Phenol Red sodium salt turns yellow.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phenol Red sodium salt Related Antibodies
Molecular Weight

376.36

Formula

C19H13NaO5S
CAS No.
Appearance

Solid

Color

Red to black

SMILES

O=S1(OC(C2=CC=C(O[Na])C=C2)(C3=CC=C(O)C=C3)C4=CC=CC=C41)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (13.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6570 mL 13.2852 mL 26.5703 mL
5 mM 0.5314 mL 2.6570 mL 5.3141 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥97.0%

SDS (393 KB)

COA (261 KB) HNMR (256 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1][2]

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6570 mL 13.2852 mL 26.5703 mL 66.4258 mL
5 mM 0.5314 mL 2.6570 mL 5.3141 mL 13.2852 mL
10 mM 0.2657 mL 1.3285 mL 2.6570 mL 6.6426 mL
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Phenol Red sodium salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenol Red34487-61-1PhenolsulfonephthaleinFluorescent DyeGlutathione PeroxidasepH indicatorhydrogen peroxideInhibitorinhibitorinhibit

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