2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Thyroid Hormone Receptor Endogenous Metabolite
  4. Protirelin

Protirelin  (Synonyms: Thyrotropin-releasing-hormone; TRH)

Cat. No.: HY-P0002 Purity: 99.51%
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Protirelin is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Protirelin Chemical Structure

Protirelin Chemical Structure

CAS No. : 24305-27-9

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5 mg USD 60 In-stock
10 mg USD 85 In-stock
25 mg USD 115 In-stock
50 mg USD 160 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of Protirelin:

Protirelin Related Antibodies

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Description

Protirelin is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

IC50 & Target

Thyroid hormone receptor
In Vivo

Protirelin (TRH) is an evolutionarily ancient neuropeptide, having its origin before the divergence of protostomes and deuterostomes, and may ancestrally have been involved in the control of postembryonic growth and reproduction[1]. The effect of the thyrotropin-releasing hormone (Protirelin, TRH), one of the hypothalamic releasing hormones, on body temperature is investigated in the rat. Protirelin, in doses of 1, 5, 10 and 20 mg/kg, is injected intraperitoneally to male Wistar rats weighing 200-250 g. Protirelin causes a temporary rise in body temperature dose-dependently. The thyroidectomized rats injected 20 mg/kg of Protirelin which induces a significant hyperthermia in the sham-operated animals, fail to show a rise in body temperature. The present results suggest that a release of thyroid hormone might participate in the hyperthermic action of Protirelin[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

362.38

Formula

C16H22N6O4
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

{pGlu}-His-Pro-NH2
Sequence Shortening

{pGlu}-HP-NH2
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (689.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7595 mL 13.7977 mL 27.5953 mL
5 mM 0.5519 mL 2.7595 mL 5.5191 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Animal Administration
[2]

Rats[2]
Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7595 mL 13.7977 mL 27.5953 mL 68.9884 mL
5 mM 0.5519 mL 2.7595 mL 5.5191 mL 13.7977 mL
10 mM 0.2760 mL 1.3798 mL 2.7595 mL 6.8988 mL
15 mM 0.1840 mL 0.9198 mL 1.8397 mL 4.5992 mL
20 mM 0.1380 mL 0.6899 mL 1.3798 mL 3.4494 mL
25 mM 0.1104 mL 0.5519 mL 1.1038 mL 2.7595 mL
30 mM 0.0920 mL 0.4599 mL 0.9198 mL 2.2996 mL
40 mM 0.0690 mL 0.3449 mL 0.6899 mL 1.7247 mL
50 mM 0.0552 mL 0.2760 mL 0.5519 mL 1.3798 mL
60 mM 0.0460 mL 0.2300 mL 0.4599 mL 1.1498 mL
80 mM 0.0345 mL 0.1725 mL 0.3449 mL 0.8624 mL
100 mM 0.0276 mL 0.1380 mL 0.2760 mL 0.6899 mL
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Protirelin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protirelin24305-27-9Thyrotropin-releasing-hormone TRHThyroid Hormone ReceptorEndogenous MetaboliteTHRInhibitorinhibitorinhibit

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