Protirelin (Synonyms: Thyrotropin-releasing-hormone; TRH)

Name: Protirelin
Brand: MedChemExpress
SKU: HY-P0002
Rating: 5.0 (3 reviews)

Cat. No.: HY-P0002 Purity: 99.51%: FAQs
Data Sheet SDS COA Handling Instructions

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Protirelin is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Protirelin Chemical Structure

CAS No. : 24305-27-9

Size	Price	Stock	Quantity
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

Other Forms of Protirelin:

Protirelin acetate In-stock
Thyrotropin-Releasing Hormone (TRH), Free Acid Get quote

3 Publications Citing Use of MCE Protirelin

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Purity & Documentation
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Description

Protirelin is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

IC₅₀ & Target

Thyroid hormone receptor

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	0.0005 μM Compound: TRH	Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay	[PMID: 26854379]
HEK293	EC₅₀	0.00052 μM Compound: TRH	Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method	[PMID: 26216015]
HEK293	EC₅₀	0.001 μM Compound: TRH	Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay	[PMID: 26854379]
HEK293	EC₅₀	0.001 μM Compound: TRH	Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method	[PMID: 26216015]
HEK293	IC₅₀	0.0026 μM Compound: TRH	Displacement of [3H]-1-MeHis-TRH from mouse TRH-R1 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method Displacement of [3H]-1-MeHis-TRH from mouse TRH-R1 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method	[PMID: 26216015]
HEK293	EC₅₀	0.003 μM Compound: TRH	Signaling (activation) potency for thyrotropin releasing hormone receptor 2 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8 Signaling (activation) potency for thyrotropin releasing hormone receptor 2 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8	[PMID: 16162016]
HEK293	EC₅₀	0.003 μM Compound: TRH	Signaling (activation) potency for thyrotropin releasing hormone receptor 1 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8; Range = 0.002-0.004 uM Signaling (activation) potency for thyrotropin releasing hormone receptor 1 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8; Range = 0.002-0.004 uM	[PMID: 16162016]
HEK293	EC₅₀	0.0031 μM Compound: TRH	Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA	[PMID: 26854379]
HEK293	EC₅₀	0.0032 μM Compound: TRH	Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA	[PMID: 26854379]
HEK293	IC₅₀	0.007 μM Compound: TRH	Displacement of [3H]-1-MeHis-TRH from mouse TRH-R2 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method Displacement of [3H]-1-MeHis-TRH from mouse TRH-R2 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method	[PMID: 26216015]
HEK293	IC₅₀	0.007 μM Compound: TRH	Displacement of [3H]-Me-His-TRH from mouse TRH-R2 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs Displacement of [3H]-Me-His-TRH from mouse TRH-R2 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs	[PMID: 26854379]
HEK293	IC₅₀	0.026 μM Compound: TRH	Displacement of [3H]-Me-His-TRH from mouse TRH-R1 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs Displacement of [3H]-Me-His-TRH from mouse TRH-R1 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs	[PMID: 26854379]

In Vivo

Protirelin (TRH) is an evolutionarily ancient neuropeptide, having its origin before the divergence of protostomes and deuterostomes, and may ancestrally have been involved in the control of postembryonic growth and reproduction^[1]. The effect of the thyrotropin-releasing hormone (Protirelin, TRH), one of the hypothalamic releasing hormones, on body temperature is investigated in the rat. Protirelin, in doses of 1, 5, 10 and 20 mg/kg, is injected intraperitoneally to male Wistar rats weighing 200-250 g. Protirelin causes a temporary rise in body temperature dose-dependently. The thyroidectomized rats injected 20 mg/kg of Protirelin which induces a significant hyperthermia in the sham-operated animals, fail to show a rise in body temperature. The present results suggest that a release of thyroid hormone might participate in the hyperthermic action of Protirelin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

362.38

Formula

C₁₆H₂₂N₆O₄

CAS No.

24305-27-9

Appearance

Solid

Color

White to off-white

Sequence

{pGlu}-His-Pro-NH2

Sequence Shortening

{pGlu}-HP-NH2

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (689.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7595 mL	13.7977 mL	27.5953 mL
5 mM	0.5519 mL	2.7595 mL	5.5191 mL
10 mM	0.2760 mL	1.3798 mL	2.7595 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.51%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (253 KB) HNMR (245 KB) LCMS (88 KB) Elemental Analysis Report (112 KB)

Handling Instructions (2659 KB)

References

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Animal Administration
^[2]

Rats^[2]
Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7595 mL	13.7977 mL	27.5953 mL	68.9884 mL
	5 mM	0.5519 mL	2.7595 mL	5.5191 mL	13.7977 mL
	10 mM	0.2760 mL	1.3798 mL	2.7595 mL	6.8988 mL
	15 mM	0.1840 mL	0.9198 mL	1.8397 mL	4.5992 mL
	20 mM	0.1380 mL	0.6899 mL	1.3798 mL	3.4494 mL
	25 mM	0.1104 mL	0.5519 mL	1.1038 mL	2.7595 mL
	30 mM	0.0920 mL	0.4599 mL	0.9198 mL	2.2996 mL
	40 mM	0.0690 mL	0.3449 mL	0.6899 mL	1.7247 mL
	50 mM	0.0552 mL	0.2760 mL	0.5519 mL	1.3798 mL
	60 mM	0.0460 mL	0.2300 mL	0.4599 mL	1.1498 mL
	80 mM	0.0345 mL	0.1725 mL	0.3449 mL	0.8624 mL
	100 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6899 mL

Protirelin Related Classifications

Inflammation/Immunology Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protirelin24305-27-9Thyrotropin-releasing-hormone TRHThyroid Hormone ReceptorEndogenous MetaboliteTHRInhibitorinhibitorinhibit

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Protirelin (Synonyms: Thyrotropin-releasing-hormone; TRH)

Customer Review

Other Forms of Protirelin:

Protirelin Related Antibodies

3 Publications Citing Use of MCE Protirelin

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Molarity Calculator

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Complete Stock Solution Preparation Table

Protirelin Related Classifications

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