Rhodamine 123 (Synonyms: RH-123; R-22420)

Name: Rhodamine 123
Brand: MedChemExpress
SKU: HY-D0816
Rating: 5.0 (15 reviews)

Cat. No.: HY-D0816 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

For research use only. We do not sell to patients.

Rhodamine 123 Chemical Structure

CAS No. : 62669-70-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 86	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 119	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 15 publication(s) in Google Scholar

15 Publications Citing Use of MCE Rhodamine 123

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
KB 3-1	IC₅₀	1.4 μM Compound: Rhodamine 123	Cytotoxicity against drug-resistant human KB31 cells after 72 hrs by MTS reduction assay Cytotoxicity against drug-resistant human KB31 cells after 72 hrs by MTS reduction assay	[PMID: 11087617]
KB 3-1	IC₅₀	1.6 μM Compound: rhodamine 123	Cytotoxicity against drug-sensitive human KB-3-1 cells after 48 hrs by MTS/PMS assay Cytotoxicity against drug-sensitive human KB-3-1 cells after 48 hrs by MTS/PMS assay	[PMID: 11720520]
KB 3-1	IC₅₀	2200 nM Compound: rhodamine123	Cytotoxicity against drug-resistant human KB-3-1 cells by MTS/PMS assay Cytotoxicity against drug-resistant human KB-3-1 cells by MTS/PMS assay	[PMID: 12350151]
KB-V1	IC₅₀	> 500 μM Compound: rhodamine 123	Cytotoxicity against multidrug-resistant human KBV1 cells after 48 hrs by MTS/PMS assay Cytotoxicity against multidrug-resistant human KBV1 cells after 48 hrs by MTS/PMS assay	[PMID: 11720520]
KB-V1	IC₅₀	> 500 μM Compound: Rhodamine 123	Cytotoxicity against multidrug-resistant human KBV1 cells after 72 hrs by MTS reduction assay Cytotoxicity against multidrug-resistant human KBV1 cells after 72 hrs by MTS reduction assay	[PMID: 11087617]
KB-V1	IC₅₀	> 500000 nM Compound: rhodamine123	Cytotoxicity against multidrug-resistant human KBV1 cells by MTS/PMS assay Cytotoxicity against multidrug-resistant human KBV1 cells by MTS/PMS assay	[PMID: 12350151]

In Vitro

1.Preparation of Rhodamine 123 working solution
1.1Preparation of the stock solution
Dissolve 1 mg Rhodamine 123 in 525 μL DMSO to obtain 5 mM of stock solution.
1.2Preparation of Rhodamine 123 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of Rhodamine 123 working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

380.82

Formula

C₂₁H₁₇ClN₂O₃

CAS No.

62669-70-9

Appearance

Solid

Color

Pink to red

Emission (Em)

529

Excitation (Ex)

507

SMILES

O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (164.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6259 mL	13.1296 mL	26.2591 mL
5 mM	0.5252 mL	2.6259 mL	5.2518 mL
10 mM	0.2626 mL	1.3130 mL	2.6259 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (279 KB) SDS (392 KB)

COA (253 KB) HNMR (261 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.

[2]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285. [Content Brief]

Kinase Assay
^[1]

Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.

[2]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6259 mL	13.1296 mL	26.2591 mL	65.6478 mL
	5 mM	0.5252 mL	2.6259 mL	5.2518 mL	13.1296 mL
	10 mM	0.2626 mL	1.3130 mL	2.6259 mL	6.5648 mL
	15 mM	0.1751 mL	0.8753 mL	1.7506 mL	4.3765 mL
	20 mM	0.1313 mL	0.6565 mL	1.3130 mL	3.2824 mL
	25 mM	0.1050 mL	0.5252 mL	1.0504 mL	2.6259 mL
	30 mM	0.0875 mL	0.4377 mL	0.8753 mL	2.1883 mL
	40 mM	0.0656 mL	0.3282 mL	0.6565 mL	1.6412 mL
	50 mM	0.0525 mL	0.2626 mL	0.5252 mL	1.3130 mL
	60 mM	0.0438 mL	0.2188 mL	0.4377 mL	1.0941 mL
	80 mM	0.0328 mL	0.1641 mL	0.3282 mL	0.8206 mL
	100 mM	0.0263 mL	0.1313 mL	0.2626 mL	0.6565 mL

Rhodamine 123 Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine 12362669-70-9RH-123 R-22420Rhodamine123Rhodamine-123RH123RH 123R22420R 22420R-22420Fluorescent DyeInhibitorinhibitorinhibit

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Rhodamine 123 (Synonyms: RH-123; R-22420)

Customer Review

Rhodamine 123 Related Antibodies

15 Publications Citing Use of MCE Rhodamine 123

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•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

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Customer Review

Rhodamine 123 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Rhodamine 123 Related Classifications

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