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  3. Fluorescent Dye
  4. Rhodamine 123

Rhodamine 123  (Synonyms: RH-123; R-22420)

Cat. No.: HY-D0816 Purity: 99.73%
COA Handling Instructions

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Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

For research use only. We do not sell to patients.

Rhodamine 123 Chemical Structure

Rhodamine 123 Chemical Structure

CAS No. : 62669-70-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 86 In-stock
25 mg USD 119 In-stock
50 mg USD 198 In-stock
100 mg USD 330 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 15 publication(s) in Google Scholar

Rhodamine 123 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

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Description

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

In Vitro

1.Preparation of Rhodamine 123 working solution
1.1Preparation of the stock solution
Dissolve 1 mg Rhodamine 123 in 525 μL DMSO to obtain 5 mM of stock solution.
1.2Preparation of Rhodamine 123 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of Rhodamine 123 working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodamine 123 Related Antibodies
Molecular Weight

380.82

Formula

C21H17ClN2O3
CAS No.
Appearance

Solid

Color

Pink to red

Emission (Em)

529
Excitation (Ex)

507
SMILES

O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (164.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6259 mL 13.1296 mL 26.2591 mL
5 mM 0.5252 mL 2.6259 mL 5.2518 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.73%

COA (253 KB) HNMR (261 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6259 mL 13.1296 mL 26.2591 mL 65.6478 mL
5 mM 0.5252 mL 2.6259 mL 5.2518 mL 13.1296 mL
10 mM 0.2626 mL 1.3130 mL 2.6259 mL 6.5648 mL
15 mM 0.1751 mL 0.8753 mL 1.7506 mL 4.3765 mL
20 mM 0.1313 mL 0.6565 mL 1.3130 mL 3.2824 mL
25 mM 0.1050 mL 0.5252 mL 1.0504 mL 2.6259 mL
30 mM 0.0875 mL 0.4377 mL 0.8753 mL 2.1883 mL
40 mM 0.0656 mL 0.3282 mL 0.6565 mL 1.6412 mL
50 mM 0.0525 mL 0.2626 mL 0.5252 mL 1.3130 mL
60 mM 0.0438 mL 0.2188 mL 0.4377 mL 1.0941 mL
80 mM 0.0328 mL 0.1641 mL 0.3282 mL 0.8206 mL
100 mM 0.0263 mL 0.1313 mL 0.2626 mL 0.6565 mL
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Rhodamine 123 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine 12362669-70-9RH-123 R-22420Rhodamine123Rhodamine-123RH123RH 123R22420R 22420R-22420Fluorescent DyeInhibitorinhibitorinhibit

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