1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. DL-O-Phosphoserine

DL-O-Phosphoserine is a normal metabolite in human biofluids and is a mixture of phosphoserine with two stereochemical structures (D-type and L-type O-phospho-serine). O-phospho-L-serine is a competitive inhibitor of serine racemase and phagocytosis. O-phospho-L-serine inhibits the enzymatic reaction by competing with the active site of serine racemase, thereby affecting the production of D-serine. O-phospho-L-serine also mimics the phosphatidylserine head group, binds to the phosphatidylserine receptor, interferes with phagocytic signal transduction, and partially blocks the phagocytosis of apoptotic cells by cells. O-phospho-L-serin can be used in the research of retinal regeneration, bone repair, and insect growth and development.

For research use only. We do not sell to patients.

DL-O-Phosphoserine Chemical Structure

DL-O-Phosphoserine Chemical Structure

CAS No. : 17885-08-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
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Based on 1 publication(s) in Google Scholar

Other Forms of DL-O-Phosphoserine:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

DL-O-Phosphoserine is a normal metabolite in human biofluids and is a mixture of phosphoserine with two stereochemical structures (D-type and L-type O-phospho-serine). O-phospho-L-serine is a competitive inhibitor of serine racemase and phagocytosis. O-phospho-L-serine inhibits the enzymatic reaction by competing with the active site of serine racemase, thereby affecting the production of D-serine. O-phospho-L-serine also mimics the phosphatidylserine head group, binds to the phosphatidylserine receptor, interferes with phagocytic signal transduction, and partially blocks the phagocytosis of apoptotic cells by cells. O-phospho-L-serin can be used in the research of retinal regeneration, bone repair, and insect growth and development[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

O-phospho-L-serin shows inhibitory activity agasint serine racemase in a concentration-dependent manner, with the enzyme reaction inhibition rate of approximately 20% at 2 mM concentration[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

O-phospho-L-serin can be used in retinal research to explore the regulatory mechanism of Müller glial cell proliferation and photoreceptor regeneration; in bone repair research, to evaluate the effects on bone cement performance and bone healing; in insect research, to identify the role of D-serine in insect growth and development. In the zebrafish light-damaged retinal model experiment, O-phospho-L-serin (20 mM, 0.5 μL; iv; injected before light treatment; single dose) can significantly reduce the number of proliferating cell nuclear antigen (PCNA)-positive Müller glial cells 51 hours after light damage, and inhibit the regeneration of cones in the light-damaged retina, but has no significant effect on the number of light-induced photoreceptor cell death[1].
In a miniature pig mandibular bone defect model experiment, a modified bone cement prepared by surgical implantation of a mixture of O-phospho-L-serin (Biozement D (2 g)-collagen-I (2.5%)-phosphoserine (50 mg)) was administered. Compared with the unmodified bone cement, the treated group had a higher absorption rate and bone regeneration rate, which effectively promoted bone healing[2].
In a silkworm growth and development experiment, O-phospho-L-serin (20 mM, 1 mL; added to feed; daily administration, from first to fifth instar larvae) delayed the development of silkworm larvae by about 6 days, significantly reduced the survival rate of larvae, and reduced the D-serine level in larvae until the cocooning stage compared with the control group[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Zebrafish retinal light damage model[1]
Dosage: 0.65% saline containing either 20 mM O-phospho-L-serine (L-SOP), 0.5 μL
Administration: Intravitreally injection via Hamilton syringe; single dose
Result: Decreased the number of PCNA-positive Müller glia 51 hours after light damage.
Inhibited the regeneration of cone photoreceptors in the light-damaged retina.
There was no significant difference in the number of light-induced photoreceptor cell deaths between the O-phospho-L-serin-injected group and the control group 24 hours after light treatment.
Molecular Weight

185.07

Formula

C3H8NO6P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OP(OCC(C(O)=O)N)(O)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 16.67 mg/mL (90.07 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.4034 mL 27.0168 mL 54.0336 mL
5 mM 1.0807 mL 5.4034 mL 10.8067 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 25 mg/mL (135.08 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.4034 mL 27.0168 mL 54.0336 mL 135.0840 mL
5 mM 1.0807 mL 5.4034 mL 10.8067 mL 27.0168 mL
10 mM 0.5403 mL 2.7017 mL 5.4034 mL 13.5084 mL
15 mM 0.3602 mL 1.8011 mL 3.6022 mL 9.0056 mL
20 mM 0.2702 mL 1.3508 mL 2.7017 mL 6.7542 mL
25 mM 0.2161 mL 1.0807 mL 2.1613 mL 5.4034 mL
30 mM 0.1801 mL 0.9006 mL 1.8011 mL 4.5028 mL
40 mM 0.1351 mL 0.6754 mL 1.3508 mL 3.3771 mL
50 mM 0.1081 mL 0.5403 mL 1.0807 mL 2.7017 mL
60 mM 0.0901 mL 0.4503 mL 0.9006 mL 2.2514 mL
80 mM 0.0675 mL 0.3377 mL 0.6754 mL 1.6886 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Product Name:
DL-O-Phosphoserine
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HY-15130
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