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  4. TMRM Perchlorate

TMRM Perchlorate  (Synonyms: T668)

Cat. No.: HY-D0984A Purity: 99.69%
SDS COA Handling Instructions

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Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

For research use only. We do not sell to patients.

TMRM Perchlorate Chemical Structure

TMRM Perchlorate Chemical Structure

CAS No. : 115532-50-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 80 In-stock
50 mg USD 280 In-stock
100 mg USD 450 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 15 publication(s) in Google Scholar

Other Forms of TMRM Perchlorate:

TMRM Perchlorate Related Antibodies

Top Publications Citing Use of Products

    TMRM Perchlorate purchased from MedChemExpress. Usage Cited in: Endocrinology. 2020 Sep 1;161(9):bqaa119.  [Abstract]

    Mitochondrial membrane potential detected by TMRE. TMRM staining reveals a decreased signal in the Immp2l knockdown granulosa cells compared to control cells, suggesting mitochondrial membrane potential depolarization. Melatonin treatment prevented the membrane depolarization in Immp2l knockdown granulosa cells.

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    • Protocol

    • Purity & Documentation

    • References

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    Description

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    In Vitro

    1.Preparation of TMRM working solution
    1.1Preparation of the stock solution
    Dissolve 1 mg TMRM in 339 μL DMSO to obtain 5 mM of stock solution.
    1.2Preparation of TMRM working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
    Note: Please adjust the concentration of TMRM working solution according to the actual situation.
    2.Cell staining
    2.1 Suspension cells (6-well plate)
    a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
    d.Wash twice with PBS, 5 minutes each time.
    e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a.Culture adherent cells on sterile coverslips.
    b.Remove the coverslip from the medium and aspirate excess medium.
    c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
    d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
    Note: If detection by flow cytometry, cells need to be resuspended before staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMRM Perchlorate Related Antibodies
    Molecular Weight

    500.93

    Formula

    C25H25ClN2O7
    CAS No.
    Appearance

    Solid

    Color

    Green to dark green

    Emission (Em)

    576
    Excitation (Ex)

    550
    SMILES

    O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OC.O=Cl(=O)([O-])=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (83.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9963 mL 9.9814 mL 19.9629 mL
    5 mM 0.3993 mL 1.9963 mL 3.9926 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.69%

    SDS (394 KB)

    COA (256 KB) HNMR (269 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay
    [1]

    Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9963 mL 9.9814 mL 19.9629 mL 49.9072 mL
    5 mM 0.3993 mL 1.9963 mL 3.9926 mL 9.9814 mL
    10 mM 0.1996 mL 0.9981 mL 1.9963 mL 4.9907 mL
    15 mM 0.1331 mL 0.6654 mL 1.3309 mL 3.3271 mL
    20 mM 0.0998 mL 0.4991 mL 0.9981 mL 2.4954 mL
    25 mM 0.0799 mL 0.3993 mL 0.7985 mL 1.9963 mL
    30 mM 0.0665 mL 0.3327 mL 0.6654 mL 1.6636 mL
    40 mM 0.0499 mL 0.2495 mL 0.4991 mL 1.2477 mL
    50 mM 0.0399 mL 0.1996 mL 0.3993 mL 0.9981 mL
    60 mM 0.0333 mL 0.1664 mL 0.3327 mL 0.8318 mL
    80 mM 0.0250 mL 0.1248 mL 0.2495 mL 0.6238 mL
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    TMRM Perchlorate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMRM115532-50-8T668T 668T-668Fluorescent DyeInhibitorinhibitorinhibit

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