Tetracaine hydrochloride (Synonyms: Amethocaine hydrochloride)

Name: Tetracaine hydrochloride
Brand: MedChemExpress
SKU: HY-B0473
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0473 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

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Tetracaine hydrochloride (Amethocaine hydrochloride) is a calcium channel protein inhibitor and blocks voltage-sensitive release of Ca²⁺ from sarcoplasmic reticulum. Tetracaine hydrochloride is mainly used topically in ophthalmology and as an antipruritic.

For research use only. We do not sell to patients.

Tetracaine hydrochloride Chemical Structure

CAS No. : 136-47-0

Size	Price	Stock	Quantity
500 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of Tetracaine hydrochloride:

Tetracaine In-stock
Tetracaine (hydrochloride) (Standard) Get quote

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-K1	IC₅₀	0.53 μM Compound: tetracaine	Inhibition of human NaV1.5 alpha subunit expressed in CHOK1 cells at -90 mV holding potential by patch clamp electrophysiological assay Inhibition of human NaV1.5 alpha subunit expressed in CHOK1 cells at -90 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
CHO-K1	IC₅₀	1.1 μM Compound: tetracaine	Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	0.11 μM Compound: tetracaine	Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	0.49 μM Compound: Tetracaine	Inhibition of recombinant human Nav1.2 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay Inhibition of recombinant human Nav1.2 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay	[PMID: 27015369]
HEK293	IC₅₀	0.52 μM Compound: Tetracaine	Inhibition of recombinant human Nav1.6 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay Inhibition of recombinant human Nav1.6 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay	[PMID: 27015369]
HEK293	IC₅₀	0.62 μM Compound: tetracaine	Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -90 mV holding potential by patch clamp electrophysiological assay Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -90 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	0.65 μM Compound: Tetracaine	Inhibition of human Nav1.5 expressed in HEK293 cells by whole cell-patch clamp method Inhibition of human Nav1.5 expressed in HEK293 cells by whole cell-patch clamp method	[PMID: 27015369]
HEK293	IC₅₀	0.74 μM Compound: Tetracaine	Inhibition of recombinant human Nav1.3 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay Inhibition of recombinant human Nav1.3 expressed in HEK293 cells preincubated for 40 mins followed by DiSBAC2 substrate addition measured after 90 mins by FRET assay	[PMID: 27015369]
HEK293	IC₅₀	33 μM Compound: tetracaine	Inhibition of human NaV1.7 F1737A mutant expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay Inhibition of human NaV1.7 F1737A mutant expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	7.51 μM Compound: Tetracaine	Inhibition of human NaV1.7 expressed in HEK293 cells by [14C]guanidinium influx assay Inhibition of human NaV1.7 expressed in HEK293 cells by [14C]guanidinium influx assay	[PMID: 21570288]

In Vitro

Tetracaine hydrochloride (Amethocaine hydrochloride) is used to alter the function of calcium release channels (ryanodine receptors) that control the release of calcium from intracellular stores. Tetracaine hydrochloride is an allosteric blocker of channel function. At low concentrations, tetracaine causes an initial inhibition of spontaneous calcium release events, while at high concentrations, tetracaine blocks release completely^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

300.82

Formula

C₁₅H₂₅ClN₂O₂

CAS No.

136-47-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(NCCCC)C=C1)OCCN(C)C.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (166.21 mM; Need ultrasonic)

DMSO : ≥ 30 mg/mL (99.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3242 mL	16.6212 mL	33.2425 mL
5 mM	0.6648 mL	3.3242 mL	6.6485 mL
10 mM	0.3324 mL	1.6621 mL	3.3242 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

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Data Sheet (267 KB) SDS (420 KB)

COA (243 KB) LCMS (84 KB)

Handling Instructions (2659 KB)

References

[1]. http://en.wikipedia.org/wiki/Tetracaine

[2]. http://www.ncbi.nlm.nih.gov/pubmed/9147318

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.3242 mL	16.6212 mL	33.2425 mL	83.1062 mL
	5 mM	0.6648 mL	3.3242 mL	6.6485 mL	16.6212 mL
	10 mM	0.3324 mL	1.6621 mL	3.3242 mL	8.3106 mL
	15 mM	0.2216 mL	1.1081 mL	2.2162 mL	5.5404 mL
	20 mM	0.1662 mL	0.8311 mL	1.6621 mL	4.1553 mL
	25 mM	0.1330 mL	0.6648 mL	1.3297 mL	3.3242 mL
	30 mM	0.1108 mL	0.5540 mL	1.1081 mL	2.7702 mL
	40 mM	0.0831 mL	0.4155 mL	0.8311 mL	2.0777 mL
	50 mM	0.0665 mL	0.3324 mL	0.6648 mL	1.6621 mL
	60 mM	0.0554 mL	0.2770 mL	0.5540 mL	1.3851 mL
	80 mM	0.0416 mL	0.2078 mL	0.4155 mL	1.0388 mL
H₂O	100 mM	0.0332 mL	0.1662 mL	0.3324 mL	0.8311 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tetracaine hydrochloride Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetracaine136-47-0AmethocaineCalcium ChannelCa2+ channelsCa channelscalciumchannelproteinvoltage-sensitivereleaseCa2+sarcoplasmicreticulumInhibitorinhibitorinhibit

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Tetracaine hydrochloride (Synonyms: Amethocaine hydrochloride)

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Tetracaine hydrochloride Related Antibodies

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Complete Stock Solution Preparation Table

Tetracaine hydrochloride Related Classifications

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