2. Anti-infection
  3. Bacterial
  4. Amifloxacin

Amifloxacin  (Synonyms: Win49375)

Cat. No.: HY-U00221 Purity: 99.23%
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Amifloxacin (Win49375) is a synthetic antibacterial agent of the quinolone class.

For research use only. We do not sell to patients.

Amifloxacin Chemical Structure

Amifloxacin Chemical Structure

CAS No. : 86393-37-5

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1 mg USD 150 In-stock
5 mg USD 375 In-stock
10 mg USD 600 In-stock
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Amifloxacin Related Antibodies

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Description

Amifloxacin (Win49375) is a synthetic antibacterial agent of the quinolone class.

IC50 & Target

Antibacterial[1]
In Vitro

Amifloxacin (WIN 49375) is active in vitro against Pseudomonas aeruginosa isolates and shows moderate activity against Staphylococcus aureus, with MICs of less than or equal to 2 μg/mL.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Amifloxacin Related Antibodies
In Vivo

Amifloxacin (WIN 49375) is highly active by the oral route, with 50% effective doses within two- to threefold of those obtained with parenteral medication. The effectiveness of Amifloxacin with various routes of medication is demonstrated with these experimental infections. When mice infected intraperitoneally with E. coli Vogel are medicated at 0.5-h postinfection subcutaneously, intravenously, or orally the ED50s for Amifloxacin are 0.6, 0.8, and 1.0 mg/kg, respectively[1]. Blood radioactivity peaks at 0.5 h after oral administration of [14C]Amifloxacin mesylate to rats at 20 mg/kg and is equivalent to 7.1±0.26 μg of Amifloxacin per mL. From 0.75 to 4 h, blood radioactivity decreases rapidly from 7.0±0.25 μg/mL to 1.2±0.12 μg/mL. Between 8 and 48 h the rate of decline in blood radioactivity slows and is more complex. At 48 h, the blood radioactivity is equivalent to 0.14±0.02 μg/mL. Blood levels of radioactivity after i.v. administration of [14C]Amifloxacin mesylate to rats at 20 mg/kg decrease from 29.1±0.85 μg/mL at 1.0 min to 14.4±0.52 μg/mL at 10 min. From 0.25 to 4 h blood radioactivity decreases from 13.0±0.42 μg/mL to 0.97±0.09 μg/mL in a log-linear manner. The rate of elimination from 4 to 24 h is slower and more complex. At 24 h, blood radioactivity is equivalent to 0.12±0.01 ug/mL[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

334.35

Formula

C16H19FN4O3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CN(NC)C2=C(C=C(F)C(N3CCN(C)CC3)=C2)C1=O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (149.54 mM; ultrasonic and adjust pH to 12 with NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9909 mL 14.9544 mL 29.9088 mL
5 mM 0.5982 mL 2.9909 mL 5.9818 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

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=
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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Animal Administration
[2]

Rats[2]
In each study, groups of eight Sprague-Dawley rats (four per sex) are dosed either orally bygavage or intravenously via the jugular vein with [14C] Amifloxacin mesylate at 20 mg/kg. All doses are prepared as solutions in distilled water. Orally medicated animals are fasted overnight before dosing. Rats in absorption studies are held in restrainers while serial blood samples are collected from the tail vein into 50 μL heparinized capillary tubes. Rats in the excretion and distribution studies are maintained in individual metabolism cages. In the distribution study, groups of rats are killed at either 0.5 or 6.0 h, blood is collected from the abdominal aorta, and tissues and organs are dissected.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.9909 mL 14.9544 mL 29.9088 mL 74.7719 mL
5 mM 0.5982 mL 2.9909 mL 5.9818 mL 14.9544 mL
10 mM 0.2991 mL 1.4954 mL 2.9909 mL 7.4772 mL
15 mM 0.1994 mL 0.9970 mL 1.9939 mL 4.9848 mL
20 mM 0.1495 mL 0.7477 mL 1.4954 mL 3.7386 mL
25 mM 0.1196 mL 0.5982 mL 1.1964 mL 2.9909 mL
30 mM 0.0997 mL 0.4985 mL 0.9970 mL 2.4924 mL
40 mM 0.0748 mL 0.3739 mL 0.7477 mL 1.8693 mL
50 mM 0.0598 mL 0.2991 mL 0.5982 mL 1.4954 mL
60 mM 0.0498 mL 0.2492 mL 0.4985 mL 1.2462 mL
80 mM 0.0374 mL 0.1869 mL 0.3739 mL 0.9346 mL
100 mM 0.0299 mL 0.1495 mL 0.2991 mL 0.7477 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Amifloxacin Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amifloxacin86393-37-5Win49375Win 49375Win-49375BacterialInhibitorinhibitorinhibit

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