Acid chrome blue K

Cat. No.: HY-W111168: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Acid chrome blue K is a metal indicator. The solution color of the LAMP (Loop-mediated isothermal amplification) reaction with ACBK (Acid chrome blue K) changes from red to blue based on a decrease in the Mg²⁺ concentration in the reaction solution.

For research use only. We do not sell to patients.

Acid chrome blue K Chemical Structure

CAS No. : 3270-25-5

Size	Price	Stock
25 g		Ask For Quote & Lead Time
50 g		Ask For Quote & Lead Time
100 g		Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

586.41

Formula

C₁₆H₉N₂Na₃O₁₂S₃

CAS No.

3270-25-5

Appearance

Solid

Color

Dark purple to black

SMILES

O=S(C1=CC=C(C(/N=N/C2=C(S(=O)(O[Na])=O)C=C3C=C(S(=O)(O[Na])=O)C=C(O)C3=C2O)=C1)O)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (85.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7053 mL	8.5265 mL	17.0529 mL
5 mM	0.3411 mL	1.7053 mL	3.4106 mL
10 mM	0.1705 mL	0.8526 mL	1.7053 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (253 KB)

Handling Instructions (2659 KB)

References

[1]. Wang X, et al. Use of a novel metal indicator to judge loop-mediated isothermal amplification for detecting the 35S promoter. Anal Bioanal Chem. 2017 Feb;409(4):881-889. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7053 mL	8.5265 mL	17.0529 mL	42.6323 mL
	5 mM	0.3411 mL	1.7053 mL	3.4106 mL	8.5265 mL
	10 mM	0.1705 mL	0.8526 mL	1.7053 mL	4.2632 mL
	15 mM	0.1137 mL	0.5684 mL	1.1369 mL	2.8422 mL
	20 mM	0.0853 mL	0.4263 mL	0.8526 mL	2.1316 mL
	25 mM	0.0682 mL	0.3411 mL	0.6821 mL	1.7053 mL
	30 mM	0.0568 mL	0.2842 mL	0.5684 mL	1.4211 mL
	40 mM	0.0426 mL	0.2132 mL	0.4263 mL	1.0658 mL
	50 mM	0.0341 mL	0.1705 mL	0.3411 mL	0.8526 mL
	60 mM	0.0284 mL	0.1421 mL	0.2842 mL	0.7105 mL
	80 mM	0.0213 mL	0.1066 mL	0.2132 mL	0.5329 mL