AF 488 maleimide 

AF 488 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 488 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules. AF488 exhibits the λ_em and λ_ex wavelength of 520 nm and 470 nm, respectively^[1][2].

734.69

C₃₁H₂₇N₄NaO₁₂S₂

Solid

Orange to red

NC(C=C1)=C(S(=O)(O[Na])=O)C2=C1C(C3=C(C(O)=O)C=C(C(NCCCCCCN4C(C=CC4=O)=O)=O)C=C3)=C(C=C5)C(O2)=C(S(=O)([O-])=O)C5=[NH2+]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Ellison CK, et al. Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules. Nat Protoc. 2019 Jun;14(6):1803-1819.  [Content Brief]

  [2]. Chakraborty S, Hu S Y, Wu S H, et al. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET[J]. PLoS One, 2015, 10(3): e0121399.