2. GPCR/G Protein Neuronal Signaling Apoptosis Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. 5-HT Receptor TNF Receptor IFNAR Reactive Oxygen Species Interleukin Related
  4. Allura Red AC

Allura Red AC  (Synonyms: FD&C RED NO. 40; CI 16035)

Cat. No.: HY-123630 Purity: 98.01%
SDS COA Handling Instructions Technical Support

Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety.

For research use only. We do not sell to patients.

Allura Red AC Chemical Structure

Allura Red AC Chemical Structure

CAS No. : 25956-17-6

Size Price Stock Quantity
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Allura Red AC:

Allura Red AC Related Antibodies

Top Publications Citing Use of Products

View All 5-HT Receptor Isoform Specific Products:

View All Isoforms
5-HT1 Receptor 5-HT2 Receptor 5-HT3 Receptor 5-HT4 Receptor 5-HT5 Receptor 5-HT6 Receptor 5-HT7 Receptor 5-HT Receptor

View All TNF Receptor Isoform Specific Products:

View All Isoforms
TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety[1][2][3].

In Vitro

Allura Red AC (1 pmol/L, 24 h) promotes 5-HT secretion, upregulates TPH1, and enhances ROS generation in BON cells (EC cell (Enterochromaffin cells) model)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Allura Red AC Related Antibodies

Western Blot Analysis[3]

Cell Line: BON cells (human enterochromaffin (EC) cell model)
Concentration: 1 pmol/L
Incubation Time: 24 h
Result: Promoted 5-HT secretion.

Real Time qPCR[3]

Cell Line: BON cells (human enterochromaffin (EC) cell model)
Concentration: 1 pmol/L
Incubation Time: 24 h
Result: Upregulated TPH1 mRNA expression.
In Vivo

Allura Red AC (100 ppm, Ad libitum intake for 12 weeks) exacerbates colitis in the DSS-induced colitis C57BL/6 mouse model and enhances inflammation through 5-HT-mediated mechanisms[3].
Allura Red AC (100 ppm (100 mg of Allura Red AC per 1 kg of diet), Ad libitum intake for 5 weeks) exacerbates colitis in the CD4+CD45RBhi T cell-induced colitis Rag1-/- mouse model and promotes T cell-mediated inflammation[3].
Allura Red AC (500-2000 mg/kg, p.o., single dose) shows no in vivo genotoxic potential in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS (HY-116282)-induced colitis C57BL/6 mouse model[3]
Dosage: 100 ppm (100 mg of Allura Red AC per 1 kg of diet)
Administration: Dietary intake, 2 weeks (84 days), followed by 3.5% DSS for 7 days
Result: Exacerbated DSS-induced colitis, reduced body weight, increased disease activity index (DAI), histological scores, and colon weight, shortened colon length, elevated colonic 5-HT, IL-1β, IL-6, and TNF-α levels.
Animal Model: CD4+CD45RBhi T cell-induced colitis Rag1-/- mouse model[3]
Dosage: 100 ppm (100 mg of Allura Red AC per 1 kg of diet)
Administration: 5 weeks, Dietary intake
Result: Promoted colitis development, increased body weight loss, disease activity index (DAI), histological scores, and colonic IL-1β, IL-6, TNF-α, and IFN-γ levels.
Animal Model: Male CD2F1 mice (8 weeks old)[2]
Dosage: 500, 1000, 2000 mg/kg
Administration: Oral gavage (p.o.), single dose
Result: Did not show genotoxicity, did not cause DNA damage, and did not induce micronuclei in bone marrow.
Molecular Weight

496.42

Formula

C18H14N2Na2O8S2
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=S(C1=CC=C2C(/N=N/C3=CC(C)=C(S(=O)(O[Na])=O)C=C3OC)=C(O)C=CC2=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (201.44 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (125.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0144 mL 10.0721 mL 20.1442 mL
5 mM 0.4029 mL 2.0144 mL 4.0288 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.0144 mL 10.0721 mL 20.1442 mL 50.3606 mL
5 mM 0.4029 mL 2.0144 mL 4.0288 mL 10.0721 mL
10 mM 0.2014 mL 1.0072 mL 2.0144 mL 5.0361 mL
15 mM 0.1343 mL 0.6715 mL 1.3429 mL 3.3574 mL
20 mM 0.1007 mL 0.5036 mL 1.0072 mL 2.5180 mL
25 mM 0.0806 mL 0.4029 mL 0.8058 mL 2.0144 mL
30 mM 0.0671 mL 0.3357 mL 0.6715 mL 1.6787 mL
40 mM 0.0504 mL 0.2518 mL 0.5036 mL 1.2590 mL
50 mM 0.0403 mL 0.2014 mL 0.4029 mL 1.0072 mL
60 mM 0.0336 mL 0.1679 mL 0.3357 mL 0.8393 mL
80 mM 0.0252 mL 0.1259 mL 0.2518 mL 0.6295 mL
100 mM 0.0201 mL 0.1007 mL 0.2014 mL 0.5036 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Allura Red AC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Allura Red AC25956-17-6FD&C RED NO. 40 CI 16035CI16035CI 16035CI-160355-HT ReceptorTNF ReceptorIFNARReactive Oxygen SpeciesInterleukin RelatedSerotonin Receptor5-hydroxytryptamine ReceptorTumor Necrosis Factor ReceptorTNFRInterferon-α/β receptorInterferon-alpha/beta receptor ILinflammatory bowel disease (IBD)intestinal barrier functionpro-inflammatory agentInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Allura Red AC
Cat. No.:
HY-123630
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (394 KB) Français - FR (394 KB) Deutsch - DE (394 KB) Norwegian - NO (394 KB) Español - ES (394 KB) Swedish - SV (394 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.