2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Ambenonium chloride

Ambenonium chloride  (Synonyms: WIN 8077 chloride)

Cat. No.: HY-100919 Purity: 95.01%
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Ambenonium (WIN 8077) chloride is an orally active and reversible inhibitor of Acetyicholinesterase (AChE) with high affinity. Ambenonium chloride inhibits human AChE with an IC50 value of 0.7 nM (hAChE).

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Ambenonium chloride Chemical Structure

Ambenonium chloride Chemical Structure

CAS No. : 115-79-7

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Based on 1 publication(s) in Google Scholar

Ambenonium chloride Related Antibodies

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Description

Ambenonium (WIN 8077) chloride is an orally active and reversible inhibitor of Acetyicholinesterase (AChE) with high affinity. Ambenonium chloride inhibits human AChE with an IC50 value of 0.7 nM (hAChE)[1][2].

IC50 & Target

IC50: 0.7 nM (hAChE), 7 μM (hBChE)[1]
In Vitro

Ambenonium chloride inhibits Acetyicholinesterase (AChE) in a rapidly reversible method, and shows strong inhibition with inhibition constant Ki of 0.12 nM against hAChE[1].
Ambenonium chloride shows inhibitory effect towards BChE with an IC50 value of 7 μM (hBChE)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ambenonium chloride Related Antibodies
In Vivo

Ambenonium chloride (6 mg/kg; p.o.; daily; 30-60 d) results an adverse effect on neuromuscular transmission in long-term administration, and induces hypersensitivity to stimulation in myasthenia gravis mice modle[3].
Ambenonium chloride (6 mg/kg; p.o.; daily; 14 d) decreases the number of AChR in motorend-plates[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Sprague Dawley rats (weight 250 g) with myasthenia gravis[3]
Dosage: 6 mg/kg
Administration: Oral gavage; daily; 14, 30, 60, 90, 360 days (Stop administration 24 h in advance)
Result: Resulted general activity decreasing and hypersensity to stimulation in rats during day 30-60, but these behaviors disappeared on day 90.
Induced degeneration and simplification of the postsynaptic folds, widening of the synaptic clefts, increased number of the postsynaptic vesicles, and reduction in the number of the AChR in the postsynaptic membrane on days 360.
Molecular Weight

608.47

Formula

C28H42Cl4N4O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCC[N+](CC)(CC)CC1=CC=CC=C1Cl)C(NCC[N+](CC)(CC)CC2=CC=CC=C2Cl)=O.[Cl-].[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (164.35 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6435 mL 8.2173 mL 16.4347 mL
5 mM 0.3287 mL 1.6435 mL 3.2869 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.6435 mL 8.2173 mL 16.4347 mL 41.0867 mL
5 mM 0.3287 mL 1.6435 mL 3.2869 mL 8.2173 mL
10 mM 0.1643 mL 0.8217 mL 1.6435 mL 4.1087 mL
15 mM 0.1096 mL 0.5478 mL 1.0956 mL 2.7391 mL
20 mM 0.0822 mL 0.4109 mL 0.8217 mL 2.0543 mL
25 mM 0.0657 mL 0.3287 mL 0.6574 mL 1.6435 mL
30 mM 0.0548 mL 0.2739 mL 0.5478 mL 1.3696 mL
40 mM 0.0411 mL 0.2054 mL 0.4109 mL 1.0272 mL
50 mM 0.0329 mL 0.1643 mL 0.3287 mL 0.8217 mL
60 mM 0.0274 mL 0.1370 mL 0.2739 mL 0.6848 mL
80 mM 0.0205 mL 0.1027 mL 0.2054 mL 0.5136 mL
100 mM 0.0164 mL 0.0822 mL 0.1643 mL 0.4109 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ambenonium chloride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ambenonium115-79-7WIN 8077WIN8077WIN-8077Cholinesterase (ChE)Myasthenia Gravismotor endplateAcetyicholinesteraseneuromuscular transmissionorally activereversibleInhibitorinhibitorinhibit

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