AMP-PNP tetralithium  (Synonyms: Adenylyl-imidodiphosphate tetralithium)

AMP-PNP tetralithium (Adenylyl-imidodiphosphate tetralithium) is a non-hydrolysable analogue of ATP and inhibits K_ATP channels^[1][2].

AMP-PNP (5 to 500 μM; Li salt) reduced the open-probability p_o of K_ATP channels and decreases the single-channel currents at high nucleotide concentrations by approximately 10%^[2].
AMP-PNP inhibits K_ATP channels of ventricular myocytes and of pancreatic β-cells both in the absence and presence of Mg²⁺ ions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

529.93

C₁₀H₁₃Li₄N₆O₁₂P₃

72957-42-7

Solid

White to off-white

O[C@H]1[C@H](N2C3=C(N=C2)C(N)=NC=N3)O[C@@H]([C@H]1O)COP(OP(NP(O[Li])(O[Li])=O)(O[Li])=O)(O[Li])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. I M Rybakowska, et al. Activities of purine converting enzymes in heart, liver and kidney mice LDLR-/- and Apo E-/. Nucleosides Nucleotides Nucleic Acids. 2018;37(6):340-347.  [Content Brief]

  [2]. S Hehl, et al. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP. Eur Biophys J. 1994;23(4):231-7.  [Content Brief]