Anti-CSCs agent-1

Cat. No.: HY-148713: FAQs
Data Sheet Handling Instructions

Molarity Calculator

Anti-CSCs agent-1 is a potent anti-CSCs agent. Anti-CSCs agent-1 inhibits cell growth and cell migration. Anti-CSCs agent-1 induces Apoptosis. Anti-CSCs agent-1 inhibits the viability of CSCs. Anti-CSCs agent-1 enhances the production of ROS in CSCs. Anti-CSCs agent-1 shows antitumor activity.

For research use only. We do not sell to patients.

Anti-CSCs agent-1 Chemical Structure

CAS No. : 2251753-58-7

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	0.7401 μM Compound: 48	Cytotoxicity activity against mouse 4T1 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against mouse 4T1 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
A-375	IC₅₀	0.8929 μM Compound: 48	Cytotoxicity activity against human A375 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human A375 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
A-375	IC₅₀	3.04 μM Compound: 48	Cytotoxicity against human A375 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay Cytotoxicity against human A375 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay	[PMID: 30433783]
A549	IC₅₀	11.56 μM Compound: 48	Cytotoxicity activity against human A549 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human A549 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
B16-F10	IC₅₀	0.6744 μM Compound: 48	Cytotoxicity activity against mouse B16F10 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against mouse B16F10 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
B16-F10	IC₅₀	1.24 μM Compound: 48	Cytotoxicity against mouse B16F10 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay Cytotoxicity against mouse B16F10 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay	[PMID: 30433783]
Lewis lung carcinoma cell line	IC₅₀	0.9066 μM Compound: 48	Cytotoxicity activity against mouse LLC cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against mouse LLC cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
MDA-MB-231	IC₅₀	3.152 μM Compound: 48	Cytotoxicity activity against human MDA-MB-231 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human MDA-MB-231 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
PANC-1	IC₅₀	1.107 μM Compound: 48	Cytotoxicity activity against human PANC1 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human PANC1 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]

In Vitro

Anti-CSCs agent-1 (compound 48) (0, 0.25, 0.5 µM) inhibits the colony formation and cell migration in A375 and B16F10 cells in a dose-dependent manner^[1].
Anti-CSCs agent-1 (0, 1, 2, 3, 4 µM; 48 h) induces apoptosis with increases in the expression of cleaved PARP, cleaved caspase-3, P-53 and Bax in a dose-dependent manner^[1].
Anti-CSCs agent-1 (0, 0.25, 0.5, 1.0, 2.0 µM; 24 h) reverses epithelial-mesenchymal transition (EMT) by significantly upregulating the expression of E-cadherin in a dose-dependent manner^[1].
Anti-CSCs agent-1 (0-10 µM; 15 days) inhibits the viability of cancer stem cells (CSCs) with IC₅₀s of 3.04, 1.24 µM for spheriod A357, B16F10 cells, respectively^[1].
Anti-CSCs agent-1 (0-2 µM; 24 h) markedly enhances the production of ROS in CSCs in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	MDA-MB-231, 4T1, A375, B16F10, PANC-1, A549, LLC cells
Concentration:	0-50 µM
Incubation Time:	48 h
Result:	Showed antiproliferative activity with IC₅₀s of 3.152, 0.7401, 0.8929, 0.6744, 1.107, 11.56, 0.9066 µM for MDA-MB-231, 4T1, A375, B16F10, PANC-1, A549, LLC cells, respectively.

Apoptosis Analysis^[1]

Cell Line:	A375, B16F10 cells
Concentration:	0, 1, 2, 3 µM
Incubation Time:	48 h
Result:	Induced cell apoptosis in a dose-dependen manner.

Western Blot Analysis^[1]

Cell Line:	A375, B16F10 cells
Concentration:	0, 1, 2, 3, 4 µM
Incubation Time:	48 h
Result:	Increased the expression of cleaved PARP, cleaved casepase-3, P-53 and Bax in a dose-dependent manner.

In Vivo

Anti-CSCs agent-1 (5 mg/kg; i.p.; daily for 15 days) shows antitumor activity in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J mice (A375 cells)^[1]
Dosage:	5 mg/kg
Administration:	I.p.; daily for 15 days
Result:	Significantly inhibited tumor growth.

Molecular Weight

713.96

Formula

C₄₄H₆₀FN₃O₄

CAS No.

2251753-58-7

SMILES

O=C(C1(CC1)C(NC2=CC=C(C=C2)F)=O)NCCNC([C@]34[C@](CC(C)(CC4)C)([H])C5=CC[C@@]([C@@]6([C@@](C(C)(C(C(C6)=C)=O)C)([H])CC7)C)([H])[C@]7(C)[C@@]5(CC3)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Liu X, et al. Synthesis and Discovery Novel Anti-Cancer Stem Cells Compounds Derived from the Natural Triterpenoic Acids. J Med Chem. 2018 Dec 13;61(23):10814-10833. [Content Brief]

Anti-CSCs agent-1 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-CSCs agent-12251753-58-7ApoptosismigrationROSantitumorEMTA375B16F10 cellsantiproliferativeapoptosisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks