AMPK beta 1 Antibody

AMPK beta 1 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 30 kDa, targeting to AMPK beta 1. It can be used for WB,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse, Rat.

AMPK beta 1: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Free

5564

Predicted band size: 30 kDa;Observed band size: 40 kDa

affinity purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

Q9Y478

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from NIH-3T3 (lane 2(20μg) or lane 3(40μg)) and Hela(lane 4(20μg) or lane 5(40μg)), using AMPK beta 1 (HY-P80542) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80542, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, FC, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human AMPK beta 1.The exact sequence is proprietary to MCE.