ERK1 Antibody

Cat. No.: HY-P80391: FAQs
Data Sheet SDS COA User Guide for Antibodies

ERK1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 43 kDa, targeting to ERK1. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	ERK1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 43 kDa, targeting to ERK1. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.
Tag	Free
Molecular Weight	Predicted band size: 43 kDa
Purity	Protein A affinity purified.
Subcellular Location	Cytoplasm, Nucleus.
Conjugation	Non-conjugated
Modification	Unmodified
Research Field	Cell Biology
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse
SwissProt ID	SwissProt: P27361 Human;SwissProt: Q63844 Mouse
Dilution Ratio	WB: 1:1,000-1:5,000 ;ICC/IF: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 Western blot analysis of extracts from M-heart (lane 1) and M-spleen (lane 2) using ERK1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application	WB, ICC/IF, IHC-P, IP, FC
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human ERK1.The exact sequence is proprietary to MCE.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (191 KB) User Guide for Antibodies (1077 KB)

ERK1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ERK1 Antibody

Biological Activity

Technical Parameters

Properties

Documentation

ERK1 Antibody Related Classifications

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