GPRC5B Antibody (YA2964)

GPRC5B Antibody (YA2964) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2964), targeting GPRC5B, with a predicted molecular weight of 45 kDa (observed band size: 45 kDa). GPRC5B Antibody (YA2964) can be used for WB, IP experiment in human, mouse, rat background.

The related gene encodes a member of the type 3 G protein-coupled receptor family. Members of The superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants.

51704

Predicted band size: 45 kDa; Observed band size: 45 kDa

Affinity Purified

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

Q9NZH0

WB: 1/500-1/1000;IP: 1/20

• 
  Western blot analysis of extracts from 3T3 (lane 2(20μg)) , A549 (lane 3(20μg)), Jurkat(lane 4(20μg)) and Daudi (lane 5(20μg)) using GPRC5B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human GPRC5B