GSK3 beta Antibody (YA744)

GSK3 beta Antibody (YA744) is a non-conjugated and Mouse origined monoclonal antibody about 47 kDa, targeting to GSK3 beta. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.

GSK3 beta: The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Free

2932

Predicted band size: 47 kDa

affinity purified

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

P49841

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from 293T (lane 1) and Jurkat (lane 2) and M-brain (lane 3) and Hela (lane 4) using GSK3 betaantibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, FC

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding tofragment of Human GSK3B.AA range:2-159.