Colored Rapid Gel Preparation Kit (12.5%) 

MCE Colored Rapid Gel Preparation Kit (12.5%) uses pre-mixed formulations of the upper gel and lower gel. Simply mix the reagents in pairs, add modified coagulation agent, and the gel will form quickly and easily. The prepared upper gel is colored, making sample loading and distinguishing different gels convenient. The kit comes with a modified polymerizing agent, APS, which has excellent stability and coagulating effects. No need to add TEMED during gel preparation.

The 125-gel version can prepare 125 mini PAGE gels (0.75 mm thickness). The prepared gels can be used for both denaturing and non-denaturing PAGE gel electrophoresis.

4℃, 1 year

Avoid repeated freeze-thaw cycles, and modified coagulation agent should be stored at -20°C if not used for a long time.

1. The upper gel prepared by this product does not concentrate samples, similar to pre-cast gels. Compared to traditional PAGE gels, it provides better protein band separation, with small proteins (e.g., 10 kDa) being clearly resolved, and the protein bands are narrower and sharper.

2. The amount of modified polymerizing agent provided is for reference. The actual amount can be adjusted based on personal habits and experience. Adding more polymerizing agent speeds up the gel formation, and less slows it down. Under the same conditions, the higher the temperature, the faster the gel forms. If the temperature is too high, consider reducing the amount of polymerizing agent.

3. Allow the gel solutions and buffers to return to room temperature before use to effectively prevent bubble formation during gel preparation.

4. After opening, the modified polymerizing agent can be stored at 4°C for at least 3 months. For long-term storage, it is recommended to aliquot and store at -20°C.

5. This product is for R&D use only, not for drug, household, or other uses.

6. For your safety and health, please wear a lab coat and disposable gloves to operate.

1. Invert all components 6–8 times to mix well before use.

2. Depending on the gel thickness needed, take equal volumes of Lower Gel Solution (2×) and Lower Gel Buffer (2×), mix well. Add the corresponding volume of the modified polymerizing agent and mix well.

3. Immediately pour the prepared lower gel into the glass plates. The liquid level should be 0.5 cm below the short glass plate edge. Add water or anhydrous ethanol to seal the liquid. Wait for the lower gel to solidify, about 10-15 min (a clear boundary between the sealing liquid and the gel indicates solidification), then pour off the top liquid.

Note: 1) The solution is prepared in excess; do not pour all of it into the plates. Leave a small amount in the tube to observe the gel state.

2) Avoid vigorous shaking while mixing, and avoid introducing air bubbles into the gel plates during pouring.

3) If the room temperature is low, solidification may take longer, so you can extend the gelation time appropriately.

4. Depending on the gel thickness needed, take equal volumes of Upper Gel Solution (2×) and Colored Upper Gel Buffer (2×), mix well; add the corresponding volume of the modified polymerizing agent and mix.

5. Immediately pour the prepared upper gel into the glass plates, insert the comb, and after the upper gel solidifies (5-10 min), remove the comb and the gel is ready for electrophoresis.

Note: 1) It is recommended to use freshly prepared electrophoresis buffer.

2) Recommended electrophoresis conditions: 150 V for 50 min or 200 V for 35 min.

1. The upper gel prepared by this product does not concentrate samples, similar to pre-cast gels. Compared to traditional PAGE gels, it provides better protein band separation, with small proteins (e.g., 10 kDa) being clearly resolved, and the protein bands are narrower and sharper.

2. The amount of modified polymerizing agent provided is for reference. The actual amount can be adjusted based on personal habits and experience. Adding more polymerizing agent speeds up the gel formation, and less slows it down. Under the same conditions, the higher the temperature, the faster the gel forms. If the temperature is too high, consider reducing the amount of polymerizing agent.

3. Allow the gel solutions and buffers to return to room temperature before use to effectively prevent bubble formation during gel preparation.

4. After opening, the modified polymerizing agent can be stored at 4°C for at least 3 months. For long-term storage, it is recommended to aliquot and store at -20°C.

5. This product is for R&D use only, not for drug, household, or other uses.

6. For your safety and health, please wear a lab coat and disposable gloves to operate.