ICAM1 Antibody (YA352)

ICAM1 Antibody (YA352) is a non-conjugated and Rabbit origined monoclonal antibody about 58 kDa, targeting to ICAM1. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human.

ICAM1: This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Free

3383

Predicted band size: 58 kDa

affinity purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P05362

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20

• 
  Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using ICAM1 (HY-P80716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80716, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human ICAM1.The exact sequence is proprietary to MCE.