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  2. Primary Antibodies
  3. Monoclonal Antibodies Recombinant Antibodies
  4. ICAM1 Antibody (YA352)

ICAM1 Antibody (YA352)

Cat. No.: HY-P80716
COA User Guide for Antibodies

ICAM1 Antibody (YA352) is a non-conjugated and Rabbit origined monoclonal antibody about 58 kDa, targeting to ICAM1. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

ICAM1 Antibody (YA352) is a non-conjugated and Rabbit origined monoclonal antibody about 58 kDa, targeting to ICAM1. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human.

Background

ICAM1: This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Tag

Free

Gene ID

3383

Synonyms
ICAM1; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54
Molecular Weight

Predicted band size: 58 kDa

Purity

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Immunology

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human

SwissProt ID

P05362

Dilution Ratio

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20

  • Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using ICAM1 (HY-P80716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80716, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application

WB, IHC-P, IP

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human ICAM1.The exact sequence is proprietary to MCE.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ICAM1 Antibody (YA352)
Cat. No.:
HY-P80716
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