IGF 1 Antibody

IGF 1 Antibody is an unconjugated, approximately 7.7/21 kDa, rabbit-derived, anti-IGF 1 polyclonal antibody. IGF 1 Antibody can be used for: WB, IHC-P, IHC-F, IF, ELISA expriments in human, mouse, rat, dog, and predicted: pig, cow, rabbit, sheep background without labeling.

The protein encoded by The related gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in The related gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to related generate mature protein.

3479

Predicted band size: 21kDa；Observed band size: 21kD

affinity purified

Secreted.

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat, Dog

Pig, Cow, Rabbit, Sheep

P05019

WB: 1:500-2000;ELISA: 1:5000-10000;IP: 1:20-100;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

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  Western blot analysis of extracts from M-Liver using IGF1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, IHC-F, ICC/IF, ELISA

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human IGF-1: 49-100/195