Phospho-Hormone sensitive lipase (Ser853) Antibody

Cat. No.: HY-P80464: FAQs
Data Sheet SDS COA User Guide for Antibodies Technical Support

Phospho-Hormone sensitive lipase (Ser853) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 117 kDa, targeting to Phospho-Hormone sensitive lipase (S853). It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Biological Activity
Technical Parameters
Properties
Documentation

Description	Phospho-Hormone sensitive lipase (Ser853) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 117 kDa, targeting to Phospho-Hormone sensitive lipase (S853). It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse, Rat.
Tag	Free
Synonyms	Hormone sensitive lipase antibody; Hormone sensitive lipase testicular isoform antibody; Hormone-sensitive lipase antibody; HSL antibody; LHS; Lipase hormone sensitive antibody; LIPE antibody; LIPS_HUMAN antibody; Phospho-Hormonesensitivelipase(S853); PhosphoHormonesensitivelipase(S853)
Molecular Weight	Predicted band size: 117 kDa;Observed band size: 90 kDa
Purity	Protein A affinity purified.
Subcellular Location	Cell membrane, cytosol, caveola, Lipid droplet.
Conjugation	Non-conjugated
Modification	Phosphorylated
Research Field	Cardiovascular
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
SwissProt ID	SwissProt: Q05469 Human;SwissProt: P54310 Mouse;SwissProt: P15304 Rat
Dilution Ratio	WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application	WB, IHC-P
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding Ser853 of Human LIPE.AA range:821-870.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (191 KB) User Guide for Antibodies (1077 KB)

Phospho-Hormone sensitive lipase (Ser853) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Phospho-Hormone sensitive lipase (Ser853) Antibody

Biological Activity

Technical Parameters

Properties

Documentation

Phospho-Hormone sensitive lipase (Ser853) Antibody Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

Request for HNMR Report