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Azure C is the product of sequential enzymatic oxidation of Methylene blue (MB) or Azure B (AB). Azure C serves as the substrate of horseradish peroxidase (HRP).

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Azure C Chemical Structure

Azure C Chemical Structure

CAS No. : 531-57-7

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Based on 1 publication(s) in Google Scholar

Azure C Related Antibodies

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Description

Azure C is the product of sequential enzymatic oxidation of Methylene blue (MB) or Azure B (AB). Azure C serves as the substrate of horseradish peroxidase (HRP)[1][2].

Molecular Weight

277.77

Formula

C13H12ClN3S
CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

[Cl-].N=1C2=CC=C(N)C=C2[S+]=C3C=C(C=CC13)NC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 1.96 mg/mL (7.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6001 mL 18.0005 mL 36.0010 mL
5 mM 0.7200 mL 3.6001 mL 7.2002 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6001 mL 18.0005 mL 36.0010 mL 90.0025 mL
5 mM 0.7200 mL 3.6001 mL 7.2002 mL 18.0005 mL
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Azure C Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Azure C531-57-7MonomethylthionineOthersInhibitorinhibitorinhibit

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HY-W143216
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