BDP 558/568 NHS ester  (Synonyms: BODIPY 558/568SE)

BDP 558/568 NHS ester is a borondipyrromethene fluorophore with emission in the yellow part of the spectrum. BDP 558/568 NHS ester is an amine reactive NHS ester, and the absorption and emission spectra of BDP 558/568 NHS ester are similar with TAMRA, BDP TMR, Cyanine3, and sulfo-Cyanine3^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Suspend sample in 0.5 M sodium bicarbonate, pH 9.0, and incubates with BDP 558/568 NHS ester for 30 min at a room temperature with frequent sonication.
2. Incubate BODIPY-sample complex with 1 mg/mL FBS for 10 min at 37℃.
3. Investigate fluorescence of BODIPY-sample complex (Ex=558 nm, Em=568 nm)^[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

443.23

C₂₀H₁₆BF₂N₃O₄S

150173-73-2

Solid

Brown to reddish brown

568

558

O=C(CCC1=CC=C2C=C3C=CC(C4=CC=CS4)=[N]3[B+3]([F-])([N-]21)[F-])ON5C(CCC5=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Vasselon T, et al. Internalization of monomeric lipopolysaccharide occurs after transfer out of cell surface CD14. J Exp Med. 1999 Aug 16;190(4):509-21.  [Content Brief]

  [2]. Yu B, Wright SD. Catalytic properties of lipopolysaccharide (LPS) binding protein. Transfer of LPS to soluble CD14. J Biol Chem. 1996 Feb 23;271(8):4100-5.  [Content Brief]