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  4. BES

BES is a secondary standard biochemical buffer with effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 ℃). BES is used in the diagnostic testing area.

For research use only. We do not sell to patients.

BES Chemical Structure

BES Chemical Structure

CAS No. : 10191-18-1

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Based on 1 publication(s) in Google Scholar

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Description

BES is a secondary standard biochemical buffer with effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 ℃). BES is used in the diagnostic testing area.

Clinical Trial
Molecular Weight

213.25

Formula

C6H15NO5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCCN(CCS(=O)(O)=O)CCO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, protect from light

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (117.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 1 mg/mL (4.69 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6893 mL 23.4467 mL 46.8933 mL
5 mM 0.9379 mL 4.6893 mL 9.3787 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (246 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.6893 mL 23.4467 mL 46.8933 mL 117.2333 mL
DMSO 5 mM 0.9379 mL 4.6893 mL 9.3787 mL 23.4467 mL
10 mM 0.4689 mL 2.3447 mL 4.6893 mL 11.7233 mL
15 mM 0.3126 mL 1.5631 mL 3.1262 mL 7.8156 mL
20 mM 0.2345 mL 1.1723 mL 2.3447 mL 5.8617 mL
25 mM 0.1876 mL 0.9379 mL 1.8757 mL 4.6893 mL
30 mM 0.1563 mL 0.7816 mL 1.5631 mL 3.9078 mL
40 mM 0.1172 mL 0.5862 mL 1.1723 mL 2.9308 mL
50 mM 0.0938 mL 0.4689 mL 0.9379 mL 2.3447 mL
60 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9539 mL
80 mM 0.0586 mL 0.2931 mL 0.5862 mL 1.4654 mL
100 mM 0.0469 mL 0.2345 mL 0.4689 mL 1.1723 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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BES Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BES10191-18-1Biochemical Assay ReagentsBufferbiochemical bufferdiagnosticInhibitorinhibitorinhibit

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