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  4. Biotin-XX hydrazide

Biotin-XX hydrazide  (Synonyms: Biotin-(AC5)2-hydrazide)

Cat. No.: HY-125501 Purity: 95.43%
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Biotin-XX hydrazide (Biotin-(AC5)2-hydrazide) is a carbonyl-reactive biotinylation reagent which contains two aminohexanoic acid spacers. Biotin-XX hydrazide has higher efficiency of avidin-binding.

For research use only. We do not sell to patients.

Biotin-XX hydrazide Chemical Structure

Biotin-XX hydrazide Chemical Structure

CAS No. : 211237-33-1

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Biotin-XX hydrazide Related Antibodies

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Description

Biotin-XX hydrazide (Biotin-(AC5)2-hydrazide) is a carbonyl-reactive biotinylation reagent which contains two aminohexanoic acid spacers. Biotin-XX hydrazide has higher efficiency of avidin-binding[1].

In Vitro

Biotin-XX hydrazide contains two aminohexanoic acid spacers which greatly enhances the efficiency of formation of the complex between the biotinylated antibody (or other biotinylated protein) and the avidin–probe conjugate, where the probe can be a fluorochrome or an enzyme[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Biotin-XX hydrazide Related Antibodies
Molecular Weight

484.66

Formula

C22H40N6O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CCCCCNC(CCCCCNC(CCCC[C@@H]1SC[C@@]([H])([C@@]1(N2)[H])NC2=O)=O)=O)NN
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (10.32 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0633 mL 10.3165 mL 20.6330 mL
5 mM 0.4127 mL 2.0633 mL 4.1266 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0633 mL 10.3165 mL 20.6330 mL 51.5826 mL
5 mM 0.4127 mL 2.0633 mL 4.1266 mL 10.3165 mL
10 mM 0.2063 mL 1.0317 mL 2.0633 mL 5.1583 mL
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Biotin-XX hydrazide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biotin-XX hydrazide211237-33-1Biotin-(AC5)2-hydrazideBiochemical Assay ReagentscarbonylbiotinylationaminohexanoicacidspacersavidinInhibitorinhibitorinhibit

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