Bis-ANS dipotassium

Name: Bis-ANS dipotassium
Brand: MedChemExpress
SKU: HY-129811
Rating: 4.5 (1 reviews)

Cat. No.: HY-129811 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Bis-ANS dipotassium is a fluorescent probe of hydrophobic protein. Bis-ANS binds to tubulin with a K_d of 2 μM. Bis-ANS dipotassium is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS). Bis-ANS dipotassium promotes LLPS at low concentrations but suppresses LLPS at high concentrations.

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Bis-ANS dipotassium Chemical Structure

CAS No. : 65664-81-5

Size	Price	Stock	Quantity
1 mg	USD 185	In-stock	Estimated Time of Arrival: December 31
5 mg		Get quote
10 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

Bis-ANS dipotassium is a fluorescent probe of hydrophobic protein. Bis-ANS binds to tubulin with a K_d of 2 μM^[1]. Bis-ANS dipotassium is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS). Bis-ANS dipotassium promotes LLPS at low concentrations but suppresses LLPS at high concentrations^[2].

In Vitro

Bis-ANS is a fluorescent molecule that has been used to probe exposed hydrophobic patches in proteins and to monitor the formation of protein aggregates. Bis-ANS is a bivalent naphthalene sulfonate with aniline moieties. Bis-ANS modulates the liquid-liquid phase separation of TDP-43 low complexity domain (LCD)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

672.85

Formula

C₃₂H₂₂K₂N₂O₆S₂

CAS No.

65664-81-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC=CC2=C(C3=C4C=CC=C(S(=O)(O[K])=O)C4=C(NC5=CC=CC=C5)C=C3)C=CC(NC6=CC=CC=C6)=C12)(O[K])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 30 mg/mL (44.59 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4862 mL	7.4311 mL	14.8622 mL
5 mM	0.2972 mL	1.4862 mL	2.9724 mL
10 mM	0.1486 mL	0.7431 mL	1.4862 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥99.0%

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Data Sheet (275 KB) SDS (393 KB)

COA (254 KB)

Handling Instructions (2659 KB)

References

[1]. P Horowitz, et al. Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly. J Biol Chem. 1984 Dec 10;259(23):14647-50. [Content Brief]

[2]. W. Michael Babinchak, et al. Small molecules as potent biphasic modulators of protein liquid-liquid phase separation. Nature Communications volume 11, Article number: 5574 (2020)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4862 mL	7.4311 mL	14.8622 mL	37.1554 mL
	5 mM	0.2972 mL	1.4862 mL	2.9724 mL	7.4311 mL
	10 mM	0.1486 mL	0.7431 mL	1.4862 mL	3.7155 mL
	15 mM	0.0991 mL	0.4954 mL	0.9908 mL	2.4770 mL
	20 mM	0.0743 mL	0.3716 mL	0.7431 mL	1.8578 mL
	25 mM	0.0594 mL	0.2972 mL	0.5945 mL	1.4862 mL
	30 mM	0.0495 mL	0.2477 mL	0.4954 mL	1.2385 mL
	40 mM	0.0372 mL	0.1858 mL	0.3716 mL	0.9289 mL