Bis-Tris

Cat. No.: HY-D0856 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5).

For research use only. We do not sell to patients.

Bis-Tris Chemical Structure

CAS No. : 6976-37-0

Size	Price	Stock	Quantity
25 g	USD 30	In-stock	Estimated Time of Arrival: December 31
50 g	USD 50	In-stock	Estimated Time of Arrival: December 31
> 100 g		Get quote

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Purity & Documentation
References
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Description

Molecular Weight

209.24

Formula

C₈H₁₉NO₅

CAS No.

6976-37-0

Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N(CCO)CCO)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 200 mg/mL (955.84 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7792 mL	23.8960 mL	47.7920 mL
5 mM	0.9558 mL	4.7792 mL	9.5584 mL
10 mM	0.4779 mL	2.3896 mL	4.7792 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (238.96 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (476 KB)

COA (247 KB) HNMR (135 KB)

Handling Instructions (2659 KB)

References

[1]. Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.

[2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]

[3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.7792 mL	23.8960 mL	47.7920 mL	119.4800 mL
	5 mM	0.9558 mL	4.7792 mL	9.5584 mL	23.8960 mL
	10 mM	0.4779 mL	2.3896 mL	4.7792 mL	11.9480 mL
	15 mM	0.3186 mL	1.5931 mL	3.1861 mL	7.9653 mL
	20 mM	0.2390 mL	1.1948 mL	2.3896 mL	5.9740 mL
	25 mM	0.1912 mL	0.9558 mL	1.9117 mL	4.7792 mL
	30 mM	0.1593 mL	0.7965 mL	1.5931 mL	3.9827 mL
	40 mM	0.1195 mL	0.5974 mL	1.1948 mL	2.9870 mL
	50 mM	0.0956 mL	0.4779 mL	0.9558 mL	2.3896 mL
	60 mM	0.0797 mL	0.3983 mL	0.7965 mL	1.9913 mL
	80 mM	0.0597 mL	0.2987 mL	0.5974 mL	1.4935 mL
	100 mM	0.0478 mL	0.2390 mL	0.4779 mL	1.1948 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bis-Tris Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bis-Tris6976-37-0Biochemical Assay ReagentsProtein degradationamine buffersInhibitorinhibitorinhibit

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