Bis-Tris hydrochloride

Cat. No.: HY-W105681 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Bis-Tris hydrochloride is a biological buffer, and can be used in gel electrophoresis separations, cell culture and bioprocessing applications.

For research use only. We do not sell to patients.

Bis-Tris hydrochloride Chemical Structure

CAS No. : 124763-51-5

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Free Sample (0.1 - 0.5 mg)		Apply Now
10 g	USD 30	In-stock	Estimated Time of Arrival: December 31
25 g	USD 47	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

Bis-Tris hydrochloride is a biological buffer, and can be used in gel electrophoresis separations, cell culture and bioprocessing applications^[1].

Molecular Weight

245.70

Formula

C₈H₂₀ClNO₅

CAS No.

124763-51-5

Appearance

Solid

Color

White to off-white

SMILES

OCCN(C(CO)(CO)CO)CCO.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (814.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0700 mL	20.3500 mL	40.7000 mL
5 mM	0.8140 mL	4.0700 mL	8.1400 mL
10 mM	0.4070 mL	2.0350 mL	4.0700 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (268 KB) SDS (393 KB)

COA (249 KB) HNMR (249 KB)

Handling Instructions (2659 KB)

References

[1]. K A Eckert, et al. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0700 mL	20.3500 mL	40.7000 mL	101.7501 mL
	5 mM	0.8140 mL	4.0700 mL	8.1400 mL	20.3500 mL
	10 mM	0.4070 mL	2.0350 mL	4.0700 mL	10.1750 mL
	15 mM	0.2713 mL	1.3567 mL	2.7133 mL	6.7833 mL
	20 mM	0.2035 mL	1.0175 mL	2.0350 mL	5.0875 mL
	25 mM	0.1628 mL	0.8140 mL	1.6280 mL	4.0700 mL
	30 mM	0.1357 mL	0.6783 mL	1.3567 mL	3.3917 mL
	40 mM	0.1018 mL	0.5088 mL	1.0175 mL	2.5438 mL
	50 mM	0.0814 mL	0.4070 mL	0.8140 mL	2.0350 mL
	60 mM	0.0678 mL	0.3392 mL	0.6783 mL	1.6958 mL
	80 mM	0.0509 mL	0.2544 mL	0.5088 mL	1.2719 mL
	100 mM	0.0407 mL	0.2035 mL	0.4070 mL	1.0175 mL