BK50164

Cat. No.: HY-150048: FAQs
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BK50164 is a potent CD73 inhibitor with an IC₅₀ value of 13.089 µM. BK50164 binds to CD99 with a K_D value of 1.5 µM. BK50164 shows antiproliferative activity. BK50164 induced Apoptosis and cell cycle arrest at Sub-G1 phase.

For research use only. We do not sell to patients.

BK50164 Chemical Structure

CAS No. : 2204291-78-9

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Description

IC₅₀ & Target

IC₅₀: 13.089 µM (CD73)^[1]

In Vitro

BK50164 (0-400 µM; 48 h) shows antiproliferative activity with IC₅₀s of 35.8, 34.28, 5.17, ≥400, ≥400 µM for A4573, TC-32, TC-71, Saos-2, U-2 OS cells, respectively^[2].
BK50164 (4-16 µM; 48 h) induces apoptosis and cell cycle arrest at Sub-G1 phase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	A4573, TC-32, TC-71, Saos-2, U-2 OS cells
Concentration:	0-400 µM
Incubation Time:	48 h
Result:	Inhibited cell viability witn IC₅₀s of 35.8, 34.28, 5.17, ≥400, ≥400 µM for A4573, TC-32, TC-71, Saos-2, U-2 OS cells, respectively.

Cell Cycle Analysis^[2]

Cell Line:	TC-32, U-2 OS cells
Concentration:	4, 8, 16 µM
Incubation Time:	48 h
Result:	Induced apoptosis in only TC-32 ES cells but not in U-2 OS cell line and induces cell cycle arrest at Sub-G1 phase.

Molecular Weight

405.72

Formula

C₁₃H₁₃ClFN₅O₇

CAS No.

2204291-78-9

SMILES

NC1=NC(Cl)=NC2=C1N=CN2[C@@H]3O[C@H](COC(C(O)=O)C(O)=O)[C@@H](O)[C@@H]3F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Roland Joseph Billedeau, et al. Ectonucleotidase inhibitors and methods of use thereof. WO2018119284A1.

[2]. Balaraman K, et al. Design, synthesis and biological evaluation of Nucleosidic CD99 inhibitors that selectively reduce Ewing sarcoma viability. Eur J Med Chem. 2023 May 5;251:115244. [Content Brief]

BK50164 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BK501642204291-78-9BK 50164BK-50164CD73Apoptosisecto-5′-nucleotidase, NT5ETC-32U-2 OS cellsantiproliferativeapoptosisSub-G1 phaseInhibitorinhibitorinhibit

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