BODIPY-FL

Cat. No.: HY-43520 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm.

For research use only. We do not sell to patients.

BODIPY-FL Chemical Structure

CAS No. : 165599-63-3

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	EC₅₀	> 30 μM Compound: 7; BODIPY FL	Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 31718946]
A-375	EC₅₀	> 30 μM Compound: 7; BODIPY FL	Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 31718946]
FaDu	EC₅₀	> 30 μM Compound: 7; BODIPY FL	Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 31718946]
HT-29	EC₅₀	> 30 μM Compound: 7; BODIPY FL	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 31718946]
MCF7	EC₅₀	> 30 μM Compound: 7; BODIPY FL	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 31718946]
NIH3T3	EC₅₀	> 30 μM Compound: 7; BODIPY FL	Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay	[PMID: 31718946]

Molecular Weight

292.09

Formula

C₁₄H₁₅BF₂N₂O₂

CAS No.

165599-63-3

Appearance

Solid

Color

Brown to red

Emission (Em)

516

Excitation (Ex)

495

SMILES

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (342.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4236 mL	17.1180 mL	34.2360 mL
5 mM	0.6847 mL	3.4236 mL	6.8472 mL
10 mM	0.3424 mL	1.7118 mL	3.4236 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (254 KB) HNMR (245 KB) CNMR (224 KB) RP-HPLC (230 KB)

Handling Instructions (2659 KB)

References

[1]. Leung K. BODIPY-FL-neutravidin-biotin-Cetuximab. 2008 Jan 15 [updated 2008 Feb 25]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. PMID: 20641313. [Content Brief]

[2]. Kurata S, et al. Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer. Nucleic Acids Res. 2001 Mar 15;29(6):E34. [Content Brief]

[3]. Fan W, et al. Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells. Bio Protoc. 2022 Nov 20;12(22):e4555. [Content Brief]

[4]. Guseva GB, et al. Design, Spectral Characteristics, and Possibilities for Practical Application of BODIPY FL-Labeled Monoterpenoid. ACS Appl Bio Mater. 2021 Aug 16;4(8):6227-6235. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4236 mL	17.1180 mL	34.2360 mL	85.5901 mL
	5 mM	0.6847 mL	3.4236 mL	6.8472 mL	17.1180 mL
	10 mM	0.3424 mL	1.7118 mL	3.4236 mL	8.5590 mL
	15 mM	0.2282 mL	1.1412 mL	2.2824 mL	5.7060 mL
	20 mM	0.1712 mL	0.8559 mL	1.7118 mL	4.2795 mL
	25 mM	0.1369 mL	0.6847 mL	1.3694 mL	3.4236 mL
	30 mM	0.1141 mL	0.5706 mL	1.1412 mL	2.8530 mL
	40 mM	0.0856 mL	0.4280 mL	0.8559 mL	2.1398 mL
	50 mM	0.0685 mL	0.3424 mL	0.6847 mL	1.7118 mL
	60 mM	0.0571 mL	0.2853 mL	0.5706 mL	1.4265 mL
	80 mM	0.0428 mL	0.2140 mL	0.4280 mL	1.0699 mL
	100 mM	0.0342 mL	0.1712 mL	0.3424 mL	0.8559 mL

BODIPY-FL Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY-FL165599-63-3Fluorescent Dyefluorescent dyeprobeprimermonoterpenoidbacteriapathogenic fungiInhibitorinhibitorinhibit

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BODIPY-FL

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Complete Stock Solution Preparation Table

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