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  4. Bromocresol green

Bromocresol green is a pH-sensitive triphenylmethane dye commonly used for the determination of protein and albumin in serum. Bromocresol green is a bio-based dye with a yellow-green to blue-green color. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in acidic solution (e.g. pH=4.15), and turns blue in basic solution (λmax=615 nm, deprotonated form). Bromocresol green is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells.

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Bromocresol green Chemical Structure

Bromocresol green Chemical Structure

CAS No. : 76-60-8

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Bromocresol green Related Antibodies

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Description

Bromocresol green is a pH-sensitive triphenylmethane dye commonly used for the determination of protein and albumin in serum. Bromocresol green is a bio-based dye with a yellow-green to blue-green color. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in acidic solution (e.g. pH=4.15), and turns blue in basic solution (λmax=615 nm, deprotonated form). Bromocresol green is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells[1][2][3][4].

In Vitro

Determination of Bromocresol Green Binding with Human Serum Albumin[1]
(1) Reagent preparation: Prepare a 0.05 M phosphate buffer or any other needed buffer, adding NaCl if necessary to maintain an ionic strength of at least 0.03. Dissolve Bromocresol Green in water and adjust the pH with NaOH to ensure it forms a divalent anionic form, creating a 0.04% stock solution.
(2) Sample preparation: Prepare a solution of human serum albumin. After determining the nitrogen content using the Kjeldahl method, dilute it with the required buffer to create a solution with an appropriate concentration of albumin. Depending on the experimental design, prepare buffers of different pH values and different concentrations of Bromocresol Green solution.
(3) Binding experiment: Under set pH conditions (e.g., pH 6.95), add a certain amount of Bromocresol Green to a solution containing a fixed concentration of human serum albumin. Measure the changes in absorbance of the solution at specific wavelengths using a spectrophotometer, and record the data. Pay special attention to changes at 615 nm, as this is where the maximum absorption difference between the free form and the albumin-bound state of Bromocresol Green occurs. Separate the free and bound indicators using ultrafiltration and measure the absorbance again.
(4) Data analysis: Analyze the absorbance data to determine the binding constant and the number of binding sites. Use the Scatchard plot analysis method to estimate the number of binding sites and their respective binding constants. Compare the experimental data with theoretical curves to evaluate the effect of different pH values on binding.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bromocresol green Related Antibodies
Molecular Weight

698.01

Formula

C21H14Br4O5S
CAS No.
Appearance

Solid

Color

Off-white to light brown

SMILES

O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2C)(C3=CC(Br)=C(O)C(Br)=C3C)C4=CC=CC=C41)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, stored under nitrogen

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (143.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4326 mL 7.1632 mL 14.3264 mL
5 mM 0.2865 mL 1.4326 mL 2.8653 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥95.0%

SDS (392 KB)

COA (264 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4326 mL 7.1632 mL 14.3264 mL 35.8161 mL
5 mM 0.2865 mL 1.4326 mL 2.8653 mL 7.1632 mL
10 mM 0.1433 mL 0.7163 mL 1.4326 mL 3.5816 mL
15 mM 0.0955 mL 0.4775 mL 0.9551 mL 2.3877 mL
20 mM 0.0716 mL 0.3582 mL 0.7163 mL 1.7908 mL
25 mM 0.0573 mL 0.2865 mL 0.5731 mL 1.4326 mL
30 mM 0.0478 mL 0.2388 mL 0.4775 mL 1.1939 mL
40 mM 0.0358 mL 0.1791 mL 0.3582 mL 0.8954 mL
50 mM 0.0287 mL 0.1433 mL 0.2865 mL 0.7163 mL
60 mM 0.0239 mL 0.1194 mL 0.2388 mL 0.5969 mL
80 mM 0.0179 mL 0.0895 mL 0.1791 mL 0.4477 mL
100 mM 0.0143 mL 0.0716 mL 0.1433 mL 0.3582 mL
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Bromocresol green Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bromocresol green76-60-8Fluorescent DyepH-sensitive triphenylmethane dyeInhibitorinhibitorinhibit

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