Calconcarboxylic acid

Cat. No.: HY-Y0700: FAQs
Data Sheet SDS COA Handling Instructions

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Calconcarboxylic acid, an azo dye, acts as a silver-ion sensitizer to stain protein in SDS-PAGE gels. Calconcarboxylic acid increases silver binding on protein bands or spots by the formation of a silver-dye complex and also increases the reducing power o

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Calconcarboxylic acid Chemical Structure

CAS No. : 3737-95-9

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Free Sample (0.1 - 0.5 mg)		Apply Now
5 g	USD 40	In-stock	Estimated Time of Arrival: December 31
10 g	USD 60	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

438.41

Formula

C₂₁H₁₄N₂O₇S

CAS No.

3737-95-9

Appearance

Solid

Color

Brown to black

SMILES

O=C(C1=C(O)C(/N=N/C2=C3C=CC=CC3=C(S(=O)(O)=O)C=C2O)=C4C=CC=CC4=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (285.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2810 mL	11.4048 mL	22.8097 mL
5 mM	0.4562 mL	2.2810 mL	4.5619 mL
10 mM	0.2281 mL	1.1405 mL	2.2810 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (253 KB) MS (72 KB)

Handling Instructions (2659 KB)

References

[1]. Jin LT, et al. Sensitive silver staining of protein in sodium dodecyl sulfate-polyacrylamide gels using an azo dye, calconcarboxylic acid, as a silver-ion sensitizer. Electrophoresis. 2004 Aug;25(15):2494-500. [Content Brief]

[2]. Hong HY, et al. Detection of proteins on polyacrylamide gels using calconcarboxylic acid. Anal Biochem. 1993 Oct;214(1):96-9. [Content Brief]

[3]. Li-Tai Jin, et al. Sensitive silver staining of protein in sodium dodecyl sulfate-polyacrylamide gels using an azo dye, calconcarboxylic acid, as a silver-ion sensitizer. Electrophoresis. 2004 Aug;25(15):2494-500. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2810 mL	11.4048 mL	22.8097 mL	57.0242 mL
	5 mM	0.4562 mL	2.2810 mL	4.5619 mL	11.4048 mL
	10 mM	0.2281 mL	1.1405 mL	2.2810 mL	5.7024 mL
	15 mM	0.1521 mL	0.7603 mL	1.5206 mL	3.8016 mL
	20 mM	0.1140 mL	0.5702 mL	1.1405 mL	2.8512 mL
	25 mM	0.0912 mL	0.4562 mL	0.9124 mL	2.2810 mL
	30 mM	0.0760 mL	0.3802 mL	0.7603 mL	1.9008 mL
	40 mM	0.0570 mL	0.2851 mL	0.5702 mL	1.4256 mL
	50 mM	0.0456 mL	0.2281 mL	0.4562 mL	1.1405 mL
	60 mM	0.0380 mL	0.1901 mL	0.3802 mL	0.9504 mL
	80 mM	0.0285 mL	0.1426 mL	0.2851 mL	0.7128 mL
	100 mM	0.0228 mL	0.1140 mL	0.2281 mL	0.5702 mL