CalFluor 488 Azide 

CalFluor 488 Azide is a water-soluble fluorogenic azide probe. CalFluor 488 Azide is activated by Cu-catalyzed or metal-free click reaction. CalFluor 488 Azide is not fluorescent until it is reacted with alkynes^[1][2]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

CalFluor 488 azide reacts with alkyne-labeled compounds to yield fluorescent triazole product (green)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

835.94

C₃₇H₄₉N₅O₁₃S₂

1798305-98-2

Solid

Orange to red

523

488

[N-]=[N+]=NC1=CC(C2=C3C=CC(C=C3OC4=C2C=CC(O)=C4)=O)=C(C=C1OCCOCC[N+](C)(CCCS(=O)([O-])=O)C)OCCOCC[N+](C)(CCCS(=O)([O-])=O)C

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• [1]. Neugebauer ME, et al. Reaction pathway engineering converts a radical hydroxylase into a halogenase. Nat Chem Biol. 2022 Feb;18(2):171-179.  [Content Brief]

  [2]. Hu J, et al. Chiral lipid bilayers are enantioselectively permeable. Nat Chem. 2021 Aug;13(8):786-791.  [Content Brief]