2. Anti-infection
  3. Bacterial Antibiotic
  4. Ceftiofur hydrochloride

Ceftiofur hydrochloride is a cell wall synthesis inhibitor that targets bacterial penicillin-binding proteins (PBPs) and has anti-inflammatory effects in endotoxemia. Ceftiofur hydrochloride exerts bactericidal effects by inhibiting the synthesis of bacterial cell wall peptidoglycan, leading to bacterial cell lysis. Ceftiofur hydrochloride also inhibits the activation of NF-κB and MAPKs, thereby reducing the secretion of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6.

For research use only. We do not sell to patients.

Ceftiofur hydrochloride Chemical Structure

Ceftiofur hydrochloride Chemical Structure

CAS No. : 103980-44-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 3 publication(s) in Google Scholar

Other Forms of Ceftiofur hydrochloride:

Ceftiofur hydrochloride Related Antibodies

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Description

Ceftiofur hydrochloride is a cell wall synthesis inhibitor that targets bacterial penicillin-binding proteins (PBPs) and has anti-inflammatory effects in endotoxemia. Ceftiofur hydrochloride exerts bactericidal effects by inhibiting the synthesis of bacterial cell wall peptidoglycan, leading to bacterial cell lysis. Ceftiofur hydrochloride also inhibits the activation of NF-κB and MAPKs, thereby reducing the secretion of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6[1][2][3].

IC50 & Target

bacterial[1]
In Vitro

Ceftiofur (1, 5, 10 mg/L; pretreatment for 1 h+LPS stimulation for 12 h) hydrochloride can significantly inhibit the secretion of TNF-α, IL-1β, and IL-6 in RAW 264.7 cells, but has no significant effect on the secretion of IL-10[2].
Ceftiofur hydrochloride (1, 5, 10 mg/L; pretreatment for 1 h+LPS stimulation for 30 min) can significantly inhibit the phosphorylation of ERK, JNK, and p38 and the nuclear translocation of NF-κB p65 in RAW 264.7 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ceftiofur hydrochloride Related Antibodies

Western Blot Analysis[2]

Cell Line: RAW 264.7 cells
Concentration: 1, 5, 10 mg/L
Incubation Time: 1 h pretreatment with Ceftiofur followed by 30 min LPS stimulation
Result: Dramatically decreased the phosphorylation levels of ERK, JNK, and p38 in Ceftiofur-treated cells compared to the LPS-treated control cells, while the total protein levels of MAPKs showed no change.

Immunofluorescence[2]

Cell Line: RAW 264.7 cells
Concentration: 1, 5, 10 mg/L
Incubation Time: 1 h pretreatment with Ceftiofur followed by 30 min LPS stimulation
Result: Nuclear translocation of NF-κB p65 induced by LPS was strongly inhibited in a dose-dependent manner in Ceftiofur-pretreated cells.
In Vivo

Ceftiofur (20 mg/kg; sc; single dose; 1 h before or 0/1 h after 30 mg/kg LPS challenge) hydrochloride significantly improves the survival rate of mice in the endotoxemia model of female C57BL/6 mice, and reduces the levels of plasma TNF-α, IL-1β, and IL-6, and increases the level of IL-10[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6 mice (18-20 g, 6-8 weeks old), LPS-induced endotoxemia model[3]
Dosage: 20 mg/kg Ceftiofur (dissolved in saline)
Administration: Subcutaneous injection, single dose, administered 1 h before or 0, 1, 4, 12 h after intraperitoneal injection of 30 mg/kg LPS.
Result: Pretreatment with 20 mg/kg Ceftiofur 1 h before LPS challenge significantly increased the survival rate of mice (70% survival at 144 h vs. 0% in LPS control group).
When administered 0 or 1 h after LPS injection, Ceftiofur still provided significant protection, but protection decreased when delayed to 4 h and was absent at 12 h.
Plasma levels of TNF-α, IL-1β, and IL-6 in Ceftiofur-pretreated mice were significantly lower than those in the LPS control group at specific time points (1 h for TNF-α, 3 h for IL-6, 12 h for IL-1β), while IL-10 levels were significantly higher at 1 h after LPS challenge.
Molecular Weight

560.02

Formula

C19H18ClN5O7S3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

[H]Cl.O=C(C(N12)=C(CSC(C3=CC=CO3)=O)CS[C@]2([H])[C@H](NC(/C(C4=CSC(N)=N4)=N\OC)=O)C1=O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 175 mg/mL (312.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7857 mL 8.9283 mL 17.8565 mL
5 mM 0.3571 mL 1.7857 mL 3.5713 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7857 mL 8.9283 mL 17.8565 mL 44.6413 mL
5 mM 0.3571 mL 1.7857 mL 3.5713 mL 8.9283 mL
10 mM 0.1786 mL 0.8928 mL 1.7857 mL 4.4641 mL
15 mM 0.1190 mL 0.5952 mL 1.1904 mL 2.9761 mL
20 mM 0.0893 mL 0.4464 mL 0.8928 mL 2.2321 mL
25 mM 0.0714 mL 0.3571 mL 0.7143 mL 1.7857 mL
30 mM 0.0595 mL 0.2976 mL 0.5952 mL 1.4880 mL
40 mM 0.0446 mL 0.2232 mL 0.4464 mL 1.1160 mL
50 mM 0.0357 mL 0.1786 mL 0.3571 mL 0.8928 mL
60 mM 0.0298 mL 0.1488 mL 0.2976 mL 0.7440 mL
80 mM 0.0223 mL 0.1116 mL 0.2232 mL 0.5580 mL
100 mM 0.0179 mL 0.0893 mL 0.1786 mL 0.4464 mL
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Ceftiofur hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ceftiofur103980-44-5BacterialAntibioticInhibitorinhibitorinhibit

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